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Diagnosis test paper for diabetic ketosis and other symptoms of relatively high ketone body

A technology of diabetic ketosis and test strips, which is applied in biological testing, material inspection products, measuring devices, etc., can solve the problems of increased culling rate of dairy cows, reduced reproductive performance, poor body condition, etc., and achieves good application prospects, good sensitivity, The effect of easy operation

Inactive Publication Date: 2013-03-27
SHANGHAI GAOFENG MEDICAL ELECTRICAL EQUIP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] (4) starvation ketosis - poor body condition and poor feed quality;
[0011] Ketosis is manifested by high blood ketones and continuous negative energy balance. In severe cases, it will lead to hyperlipidemia and fatty liver, decreased milk production and milk quality, reduced reproductive performance, and endocrine disorders, which will lead to an increase in the culling rate of dairy cows. cause serious economic losses

Method used

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  • Diagnosis test paper for diabetic ketosis and other symptoms of relatively high ketone body
  • Diagnosis test paper for diabetic ketosis and other symptoms of relatively high ketone body
  • Diagnosis test paper for diabetic ketosis and other symptoms of relatively high ketone body

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] The preparation process that the total volume of the first phase enzyme solution liquid is 100mL is as follows:

[0054] 1) Dissolve 5g of gum arabic;

[0055] 2) Dissolve 500mg of bovine serum albumin;

[0056] 3) Dissolve 300mg of oxalic acid;

[0057] 4) Dissolve NAD + 0.5g, NADP + 0.2g;

[0058] 5) Dissolve diaphorase, 10000iu;

[0059] 6) Dissolve 100000iu of 3-hydroxybutyrate dehydrogenase;

[0060] 7) Add 50mmol / L Tris-malonic acid buffer solution with pH 8.0 to 100ml;

[0061] First phase solution impregnation:

[0062]After the solution is prepared, add the dipping machine, install a roll of whatman 3MM filter paper, set the drying temperature to 30°C, set the paper speed to 3.0mm / s, and start impregnating the paper. After the solution is soaked, the filter paper is placed in the drying box Leave for 1 hour for complete drying;

[0063] Second phase solution preparation:

[0064] Dissolve 250mg of nitro blue tetrazolium chloride; (that is, the concent...

Embodiment 2

[0074] The preparation process that the total volume of the first phase enzyme solution liquid is 100mL is as follows:

[0075] 8) Dissolve 5g of gum arabic;

[0076] 9) Dissolve 500mg of bovine serum albumin;

[0077] 10) Dissolve 300mg of oxalic acid;

[0078] 11) Dissolve NAD + 0.5g, NADP + 0.2g;

[0079] 12) Dissolve diaphorase, 10000iu;

[0080] 13) Dissolve 100000iu of 3-hydroxybutyrate dehydrogenase;

[0081] 14) Add 50mmol / L Tris-malonic acid buffer solution with pH 8.0 to 100ml;

[0082] First phase solution impregnation:

[0083] After the solution is prepared, add the dipping machine, install a roll of whatman 3MM filter paper, set the drying temperature to 30°C, set the paper speed to 3.0mm / s, and start impregnating the paper. After the solution is soaked, the filter paper is placed in the drying box Leave for 1 hour for complete drying;

[0084] Second phase solution preparation:

[0085] Dissolve 250 mg of nitro blue tetrazolium chloride;

[0086] Phen...

Embodiment 3

[0095] The preparation process that the total volume of the first phase enzyme solution liquid is 100mL is as follows:

[0096] 1) Dissolve 5g of gum arabic;

[0097] 2) Dissolve 500mg of bovine serum albumin;

[0098] 3) Dissolve 300mg of oxalic acid;

[0099] 4) Dissolve NAD + 0.5g, NADP + 0.5g;

[0100] 5) Dissolve diaphorase, 10000iu;

[0101] 6) Dissolve 100000iu of 3-hydroxybutyrate dehydrogenase;

[0102] 7) Add 80mmol / L Tris-malonic acid buffer solution with pH 8.7 to 100ml;

[0103] First phase solution impregnation:

[0104] After the solution is prepared, add the dipping machine, install a roll of whatman 3MM filter paper, set the drying temperature to 30°C, set the paper speed to 3.0mm / s, and start impregnating the paper. After the solution is soaked, the filter paper is placed in the drying box Leave for 1 hour for complete drying;

[0105] Second phase solution preparation:

[0106] Dissolve 250 mg of nitro blue tetrazolium chloride;

[0107] Phenazine...

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Abstract

The invention relates to diagnosis test paper for diabetic ketosis and other symptoms of relatively high ketone body. The diagnosis test paper comprises the following reagents: (1) first-phase immersion liquid which comprises a stabilizer and a protective agent, bovine serum albumin, oxalic acid, NAD<+>, NADP<+>, diaphorase, 3-hydroxybutyrate dehydrogenase and buffer solution; and (2) second-phase immersion liquid which comprises NBT, PMS and an organic solvent. The diagnosis test paper is prepared by the following steps of: soaking filter paper in the first-phase immersion liquid, sucking redundant immersion liquid and quickly and warmly drying the soaked filter paper at the temperature of between 20 and 50 DEG C; soaking the dried filter paper in the second-phase immersion liquid and drying at the temperature of between 20 and 50 DEG C again to obtain base paper of the diagnosis test paper; and sticking the base paper on a plastic base and cutting into pieces to obtain the diagnosis reagent strips. The diagnosis test paper can be assembled into various detection devices, has the advantages of convenient operation, fast detection, good sensitivity, high accuracy and the like, can semiquantitatively detect the 3-hydroxybutyric acid content of milk, urine and blood, and has good application prospect.

Description

technical field [0001] The invention belongs to the field of clinical detection of diabetic ketosis and other high ketone body symptoms, in particular to a diagnostic test paper for diabetic ketosis and other high ketone body symptoms. Background technique [0002] 3-Hydroxybutyric acid (3-Hydroxybutyric acid) is one of the end products of fat metabolism, accounting for about 78% of ketone bodies, while acetoacetic acid and acetone account for 22%. Molecular formula: C 4 h 8 o 3 , the structural formula is as follows: [0003] [0004] Under normal circumstances, the concentration of ketone bodies is basically constant, but in the early stage of diabetic ketosis and other symptoms of high ketone bodies, 3-hydroxybutyric acid can increase significantly, and there is no obvious change in acetoacetate at this time. In the recovery period of ketosis, when 3-hydroxybutyric acid drops rapidly, acetoacetic acid remains elevated or decreases slowly for a certain period of time...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/64G01N33/531G01N21/78
Inventor 王福进管利丰孙大尼
Owner SHANGHAI GAOFENG MEDICAL ELECTRICAL EQUIP
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