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Method for extracting polysaccharide from fresh hedgehog hydnum

A technology of Hericium erinaceus and polysaccharides, applied in the field of polysaccharide extraction, can solve the problems of large consumption, low extraction efficiency, solvent residue, etc., achieve the effects of reducing pollution, simple and feasible process, and saving energy

Inactive Publication Date: 2010-12-22
吴茂玉 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

This method has low extraction efficiency, consumes a large amount of solvent, and is difficult to carry out industrial production. The resulting polysaccharide product has low purity, not only contains a large amount of impurities such as pigments and proteins, but also has unavoidable solvent residues, which limits the application of Hericium erinaceus polysaccharides.

Method used

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Examples

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Embodiment

[0010] A kind of described concrete implementation example of utilizing fresh Hericium erinaceus to extract polysaccharides is as follows:

[0011] (1) Choose intact and non-rotten fresh Hericium erinaceus, crush it with a crusher, add 1.5 times the quality of water, and then process it twice through a colloid mill to obtain Hericium erinaceus slurry.

[0012] (2) Add 6 times the mass of water to the obtained Hericium erinaceus slurry, stir evenly, then extract in a water bath at 60°C for 2 hours, filter and collect the filtrate.

[0013] (3) Adjust the pH value of the filtrate to 4.5 with dilute hydrochloric acid, adopt HD-3 resin column, and perform chromatography at a flow rate of 5BV / h, and collect 6BV of the eluate.

[0014] (4) Regulating eluent pH with dilute NaOH is 7.0, and adopting molecular weight cut-off earlier is 50KD ultrafiltration membrane ultrafiltration, collects filtrate, then is 10KD ultrafiltration membrane ultrafiltration with molecular weight cut-off, c...

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PUM

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Abstract

The invention relates to a method for extracting polysaccharide from fresh hedgehog hydnum, which comprises the following steps of: leaching the fresh hedgehog hydnum in water at the temperature of between 55 and 65 DEG C for 2 hours, and filtering to obtain filter liquor; regulating a pH value of the filter liquor to between 4.0 and 4.5; performing chromatography on a HD-3 resin column for decoloring and deproteinizing, and performing ultrafiltration grading by using ceramic ultrafiltration membranes of which the molecular weight cutoff is 50 KD and 10 KD; and concentrating intermediate trapped fluid and spray-drying to prepare the polysaccharide, wherein the total recovery is 0.96 percent, and the purity is between 71 and 83 percent. The method has the advantages of high purity of the obtained hedgehog hydnum polysaccharide, simple and feasible process, no need of a drying process or an organic solvent, recycled resin, energy conservation, pollution reduction, and particular suitability for continuous industrial production.

Description

Technical field: [0001] The invention relates to a method for extracting polysaccharides from fresh Hericium erinaceus. Background technique: [0002] Hericium erinaceus is a rare edible and medicinal fungus, which has the effects of benefiting the five internal organs, aiding digestion, nourishing and anti-cancer. Its main active ingredient polysaccharide is a glucan mixture whose main chain is connected by β-1,3 glycosidic bonds and branched chains are connected by β-1,6 glycosidic bonds. A large number of studies have shown that Hericium erinaceus polysaccharides have anti-tumor, enhanced humoral immune function, lowering blood sugar, lowering blood fat, anti-coagulation, anti-mutation, etc. Hericium erinaceus polysaccharide has great medical value. [0003] Existing extraction methods mostly dry Hericium erinaceus with water, dilute acid, dilute alkali leaching, and the method of organic solvent precipitation. This method has low extraction efficiency, consumes a larg...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
Inventor 吴茂玉赵岩于洪涛肖丽霞葛邦国宋烨和法涛马超
Owner 吴茂玉
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