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Method for extracting active ingredients from sea horse

An extraction method and technology of active ingredients, applied in the field of extracting active ingredients of hippocampus, can solve the problems of low yield of extracts, achieve the effect of increasing the yield and enhancing the health of the body

Inactive Publication Date: 2010-12-29
海南壹号药业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0016] The purpose of the present invention is to provide a method for extracting active ingredients of hippocampus, which solves the problem of low extract yield in the prior art

Method used

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  • Method for extracting active ingredients from sea horse
  • Method for extracting active ingredients from sea horse
  • Method for extracting active ingredients from sea horse

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Dry the live hippocampus and mash it, add water 20 times the mass of the hippocampus to the mashed hippocampus, and heat it to 50°C, then add trypsin with 1% of the mass of the hippocampus to hydrolyze until the pH of the solution no longer decreases, and then filter To obtain the hydrolyzate, reflux the filter residue with 60% alcohol at a temperature of 60°C for 2 h to obtain an alcohol extract; Concentrate with a rotary evaporator so that the mass of the concentrated dry matter accounts for 40% of the mass of the mixed solution to obtain a concentrated solution; the concentrated solution is spray-dried at an inlet air temperature of 160°C to obtain the active ingredient of hippocampus.

Embodiment 2

[0037] Dry the live hippocampus and mash it, add water 20 times the mass of the hippocampus to the mashed hippocampus, and heat it to 55°C, then add trypsin with 1% of the mass of the hippocampus to hydrolyze until the pH of the solution no longer decreases, and then filter To obtain the hydrolyzate, reflux the filter residue with 60% alcohol at a temperature of 65°C for 2.5 hours to obtain an alcohol extract; Concentrate with a rotary evaporator so that the mass of the concentrated dry matter accounts for 45% of the mass of the mixed solution to obtain a concentrated solution; the concentrated solution is spray-dried at an inlet air temperature of 170°C to obtain the active ingredient of hippocampus.

Embodiment 3

[0039] Dry the live hippocampus and mash it, add water 20 times the mass of the hippocampus to the mashed hippocampus, and heat it to 53°C, then add trypsin with 1% of the mass of the hippocampus to hydrolyze until the pH of the solution no longer decreases, and then filter To obtain the hydrolyzate, reflux the filter residue with 60% alcohol at a temperature of 63°C for 2.2 hours to obtain an alcohol extract; Concentrate with a rotary evaporator so that the mass of the concentrated dry matter accounts for 42% of the mass of the mixed solution to obtain a concentrated solution; the concentrated solution is spray-dried at an inlet air temperature of 165°C to obtain the active ingredient of hippocampus.

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Abstract

The invention discloses a method for extracting active ingredients from a sea horse. The method comprises the following steps of: drying the sea horse, crushing the sea horse, adding water to the crushed sea horse, and heating the liquid; adding trypsase into the liquid to hydrolyze the crushed sea horse, filtering the solution to obtain a hydrolyzate, and refluxing the filter residue in alcohol to obtain an alcohol immersion liquid; mixing the hydrolyzate and the alcohol immersion liquid, and concentrating the mixture with a rotary evaporator to obtain a concentrated solution; and performing spray drying on the concentrated solution to obtain the active ingredients of the sea horse. By the method of combining enzymatic extraction and ethanol extraction, the yield of the extract is improved to 148.45 percent, so that the treatment effect of traditional Chinese medicine, namely the sea horse, is better and the sea horse improves the health of people. The problem of low yield of extract in the prior art is solved.

Description

technical field [0001] The invention belongs to the technical field of food biological extraction, and relates to a method for extracting active ingredients of hippocampus. Background technique [0002] Hippocampus is a precious marine Chinese herbal medicine in my country, and it is a traditional high-end nourishing food. It is recorded in ancient medical books of all dynasties and all previous editions of Chinese Pharmacopoeia (Chinese medicine part). "Compendium of Materia Medica" records: "Hippocampus, the main dystocia and blood gas pain; warm water and dirty, aphrodisiac; sand lumps, treatment of sores and pain." Pharmacological experiments have proved that the hippocampus extract has androgen-like effects, and the ethanol extract of Kleiner's hippocampus can prolong the estrus period of mice. Resolving knots and reducing swelling, treating tumors, boils and swollen toxins. Hormone-like effect, anti-aging effect, antithrombotic effect and sedative effect. [0003] T...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/56A61P5/26A61P7/02A61P25/20A61P29/00A61P35/00A61P37/04A61P39/06A61K35/60
Inventor 易美华陈成波袁学会陈政
Owner 海南壹号药业有限公司
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