Fungus capable of degrading multiple types of dyes under non-sterilization condition and application thereof
A kind of fungus and type of technology, which is applied in the direction of fungi, microorganism-based methods, textile industry wastewater treatment, etc., can solve the problems of widely varying application costs, achieve wide pH adaptability, reduce treatment costs, and require low nutrients Effect
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[0019] 1. Isolation and identification of strain Trichaptum abietinum 1302BG
[0020] The soil comes from the thunder bamboo forest in Lin'an, Hangzhou. Weigh 1 g of the soil, put it into a sterilized Erlenmeyer flask, add 9 mL of sterile water, and shake it for 10 minutes. Then, the bacterial suspension was diluted to 10 -1 , 10 -2 , 10 -3 , 10 -4 , four gradients. take 10 -2 , 10 -3 , 10 -4 200 μL of suspensions of three concentrations were inoculated on a plate of tiger bengal solid medium, and cultured at 28°C for 2 to 7 days. According to the colony morphology and anatomical observation under a microscope, they were preliminarily classified and preserved, and a total of 96 strains of fungi were obtained. The above-mentioned 96 strains of fungi were inoculated into PDA+aniline blue (100mgL -1 ) plate, cultured at 28°C for 7 days, observed and recorded the decolorization effect of each fungus, and obtained 15 strains of fungi with decolorization ability. Among them...
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