Fungus capable of degrading multiple types of dyes under non-sterilization condition and application thereof

A kind of fungus and type of technology, which is applied in the direction of fungi, microorganism-based methods, textile industry wastewater treatment, etc., can solve the problems of widely varying application costs, achieve wide pH adaptability, reduce treatment costs, and require low nutrients Effect

Inactive Publication Date: 2011-01-19
NANJING UNIV
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  • Abstract
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Problems solved by technology

Although the existing solutions can partially overcome the decline in the decolorization ability of fungi caused by other microbial contamination, they are still far from the actual application cost

Method used

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  • Fungus capable of degrading multiple types of dyes under non-sterilization condition and application thereof
  • Fungus capable of degrading multiple types of dyes under non-sterilization condition and application thereof
  • Fungus capable of degrading multiple types of dyes under non-sterilization condition and application thereof

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Experimental program
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Effect test

Embodiment Construction

[0019] 1. Isolation and identification of strain Trichaptum abietinum 1302BG

[0020] The soil comes from the thunder bamboo forest in Lin'an, Hangzhou. Weigh 1 g of the soil, put it into a sterilized Erlenmeyer flask, add 9 mL of sterile water, and shake it for 10 minutes. Then, the bacterial suspension was diluted to 10 -1 , 10 -2 , 10 -3 , 10 -4 , four gradients. take 10 -2 , 10 -3 , 10 -4 200 μL of suspensions of three concentrations were inoculated on a plate of tiger bengal solid medium, and cultured at 28°C for 2 to 7 days. According to the colony morphology and anatomical observation under a microscope, they were preliminarily classified and preserved, and a total of 96 strains of fungi were obtained. The above-mentioned 96 strains of fungi were inoculated into PDA+aniline blue (100mgL -1 ) plate, cultured at 28°C for 7 days, observed and recorded the decolorization effect of each fungus, and obtained 15 strains of fungi with decolorization ability. Among them...

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Abstract

The invention belongs to the technical field of industrial microorganism, and in particular relates to fungus capable of efficiently degrading multiple types of dyes under non-sterilization condition. Microorganism from the soil of a phyllostachys praecox forest in Hangzhou Lin'an is separated on a PDA culture medium by a gradient dilution method and is subjected to laboratory screening, so that the important fungus-trichaptum abietinum 1302BG capable of efficiently degrading multiple types of dyes under the non-sterilization condition is obtained. The strain can decolorize multiple types of dyes, has wide pH adaptability, has low requirement on nutrient components of the culture medium, and still has efficient decolorization under the non-sterilization condition even the condition of artificially added with certain other microorganism pollution source. Dye solution decolorized by the strain does not have toxicity or the toxicity is largely reduced. The excellent characteristics show that the strain has high treatment and application capability for industrial dye wastewater.

Description

1. Technical field [0001] The invention belongs to the technical field of industrial microbes, and in particular relates to a fungus capable of degrading various types of dyes under the conditions of non-sterilization and low-cost and simple nutrient medium. 2. Background technology [0002] Synthetic dyes are widely used in textile, food, medicine, leather, cosmetics and other industries. It is estimated that the total production of dyestuffs per year is about 7×10 5 Tons, my country's annual output has reached 150,000 tons, of which about 10% to 15% of dyes will be directly discharged into the environment along with wastewater. There are many dyes used in industry, such as azo, triphenylmethane, anthraquinone and so on. Many dyes and their decomposition products are toxic and can cause cancer and distortion. If printing and dyeing wastewater is discharged directly into rivers without treatment, it will inevitably cause serious environmental pollution. [0003] Syntheti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C02F3/34C12R1/645C02F103/30
Inventor 韩国民田兴军王从彦贾永何兴兵张鹏郭鹏陈展慧冯晓光王方圆
Owner NANJING UNIV
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