Heat resistant beta-amylase-trehalose synthase fusion enzyme, expression gene of heat resistant beta-amylase-trehalose synthase fusion enzyme, engineering bacterium secreting fusion enzyme, and application

Abstract

The invention provides a heat resistant beta-amylase-trehalose synthase fusion enzyme, an expression gene of the heat resistant beta-amylase-trehalose synthase fusion enzyme, an engineering bacterium secreting the fusion enzyme and application, and belongs to the technical field of genetic engineering. The heat resistant beta-amylase-trehalose synthase fusion enzyme comprises an amino acid sequence with SEQ ID No.1 and another amino acid sequence, and the similarity of the another amino acid sequence to the amino acid sequence with SEQ ID No.1 is 80%-100%. The expression gene of the heat resistant beta-amylase-trehalose synthase fusion enzyme comprises an amino acid sequence with SEQ ID No.2 and another amino acid sequence, and the similarity of the another amino acid sequence to the amino acid sequence with SEQ ID No.2 is 80%-100%. The obtained beta-amylase-trehalose synthase fusion enzyme has a heat resistant property, directly converts amylose into trehalose at the higher temperature, saves the complicated steps of separating and purifying endoenzyme, simplifies the production process of the trehalose, and reduces the production cost.

Description

technical field [0001] The invention relates to a heat-resistant β-amylase-trehalose synthase fusion enzyme, an expression gene thereof, an engineering bacterium that secretes the fusion enzyme and its application, and belongs to the technical field of genetic engineering. Background technique [0002] Trehalose (trehalose) is also known as glucosylose, mushroom sugar, etc., and its chemical name is α-D-glucopyranosyl α-D-glucopyranoside. It is composed of 2 glucose molecules through α,α-1,1- A natural non-reducing disaccharide composed of glycosidic bonds, the molecular formula is C 12 h 22 o 11 2H 2 O, molecular weight 378.33. Trehalose is widely found in many edible animals, plants and microorganisms in nature, such as mushrooms, seaweed, beans, shrimp, bread, beer and yeast fermented foods have a high content of trehalose. Trehalose has non-specific protective effects on a variety of organisms. Scientists have discovered that trehalose has created a variety of mira...

AI Technical Summary

Problems solved by technology

[0006] The present invention aims at the problem that in the prior art, in the process of synthesizing trehalose with starch as a substrate, it is necessary to use wild bacteria or engineered bacteria to ferment a large number of bacteria, and then pass tedious protein separation and purification steps to obtain enough enzymes and high production costs. , providing thermostable β-amylase-trehalose synthase fusion enzyme, its expression gene, and engineering bacteria and applications that secrete the fusion enzyme, specifically providing a thermostable β-amylase-trehalose synthase fusion enzyme that can be secreted and expressed Genetically engineered bacteria and using the engineered bacteria to ferment and produce enzymes for the preparation of trehalose

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