Fermentation preparation method, and separation and purification method of heat-resistant and acid-resistant glucose oxidase
A glucose oxidase and heat-resistant technology, applied in the field of microorganisms, can solve the problems of difficult promotion and use of glucose oxidase, limitation of the scope of use of glucose oxidase, and relatively little research on glucose oxidase, so as to save manpower and financial resources and effectively Facilitate transportation, easy extraction and separation
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Embodiment 1
[0047] A fermentation preparation method for heat-resistant and acid-resistant glucose oxidase produced by Paecilomyces thermophila, the specific steps are as follows:
[0048] (1) Streak and activate Paecilomyces thermophila species on the PDA activation medium plate, and place the PDA activation medium plate in a constant temperature incubator at 35° C. for 50 h to obtain Paecilomyces thermophila filaments;
[0049] (2) Scrape the Paecilomyces thermophila filaments prepared in step (1) with an inoculation loop and inoculate them on the Paecilomyces thermophila spore medium, and place the Paecilomyces thermophila spore medium at 35°C Cultivate for 3 days to obtain spores;
[0050] (3) The prepared spores in the step (2) are cleaned with 0.75% sterile saline containing 0.01% (v / v) Tween 80, and the cleaned spores are put into 50mL sterile saline and Place in a conical flask with sterile glass beads and fully shake for 40 minutes. After the spores are fully dispersed, prepare ...
Embodiment 2
[0063] A fermentation preparation method for heat-resistant and acid-resistant glucose oxidase produced by Paecilomyces thermophila, the specific steps are as follows:
[0064] (1) Streak and activate Paecilomyces thermophila species on the PDA activation medium plate, and place the PDA activation medium plate in a constant temperature incubator at 30°C for 48 hours of activation culture to obtain Paecilomyces thermophila filaments;
[0065] (2) Use the inoculation loop to scrape the prepared Paecilomyces thermophila filaments in step (1) and inoculate them on the Paecilomyces thermophila spore medium, and place the Paecilomyces thermophila spore medium at 30°C Under culture for 2 days, the spores are obtained;
[0066](3) The prepared spores in the step (2) are cleaned with 0.75% sterile saline containing 0.01% (v / v) Tween 80, and the cleaned spores are put into 50mL sterile saline and Place in a conical flask with sterile glass beads and fully shake for 30 minutes. After th...
Embodiment 3
[0079] A fermentation preparation method for heat-resistant and acid-resistant glucose oxidase produced by Paecilomyces thermophila, the specific steps are as follows:
[0080] (1) Streak and activate Paecilomyces thermophila species on a PDA activation medium plate, and place the PDA activation medium plate in a constant temperature incubator at 40°C for 72 hours of activation culture to obtain Paecilomyces thermophila filaments;
[0081] (2) Use the inoculation loop to scrape the prepared Paecilomyces thermophila filaments in step (1) and inoculate them on the Paecilomyces thermophila spore medium, and place the Paecilomyces thermophila spore medium at 40°C Under culture for 4 days, the spores are obtained;
[0082] (3) The prepared spores in the step (2) are cleaned with 0.75% sterile saline containing 0.01% (v / v) Tween 80, and the cleaned spores are put into 55mL sterile saline and Place in a conical flask with sterile glass beads and fully shake for 45 minutes. After the...
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