Sphingosine monad DX-T3-03 strain and extracting method thereof
A sphingomonas and extraction method technology are applied in the field of sphingomonas DX-T3-03 strain and its extraction to achieve the effects of good degradability, environmental friendliness and low nutritional requirements
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[0032] Example 1
[0033] (1) Take the soil from the tailing dam of a certain mine to make a soil suspension and inoculate it in a zinc-containing inorganic salt medium with phenol as the sole carbon source at an input amount of 5 wt%. The inorganic salt medium is (NH 4 ) 2 SO 4 10.0g, KH 2 PO 4 7.5g, Na 2 HPO 4 10.0g, FeCl 3 0.0125g, MgSO 4 ·7H 2 O 0.015g, 1000mL distilled water, pH=6.5~7.0. At the same time add gradient concentration of filter bacteria phenol and filter bacteria Zn 2+ Solution so that the medium contains both phenol and Zn 2+ And the concentrations of the two substances increased sequentially, phenol 500, 600, 700, 800, 900, 1000 mg / L, Zn 2+ Concentrations 0.5, 1.0, 1.5, 2.0, 2.5, 3.0, 3.5, 4.0 mM / L, the interval of changing the concentration is 6 to 7 days, and the acclimatization culture is carried out at 30 to 35°C and an oscillation rate of 150r / min for 2 months;
[0034] (2) Take the sample after 2 months of acclimatization and let it stand for 30 minutes...
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