Microwave enzyme method-assisted method for extracting crude solanesol from potato leaf
An auxiliary extraction and potato technology, which is applied in the separation/purification of hydroxyl compounds, organic chemistry, fermentation, etc., can solve the problems that lye is easy to cause environmental pollution, etc., and achieve significant economic benefits and less waste liquid discharge.
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[0024] Example 1
[0025] The present invention provides a method for extracting crude solanesol by microwave enzymatic method using potato leaves as raw materials, which comprises the following steps:
[0026] 1) Crush dried potato leaves, pass a 40-mesh sieve, add distilled water, accurately weigh 40 grams of potato leaves, add 500ml of distilled water, ultrasonically wash for 60 minutes under the conditions of ultrasonic power 250W, frequency 40KHz and temperature 60℃, and then filter. Get substance A;
[0027] 2) Add 800ml of cellulase solution with a mass concentration of 0.002g / ml prepared with a citric acid buffer with a pH of 4.4 to substance A, at a microwave power of 50W, a frequency of 2450MHz, a temperature of 30℃, and a stirring speed of 120r / min Under the conditions, microwave-assisted enzymatic hydrolysis was performed for 15 minutes, and centrifuged for 10 minutes at 4000 rpm, and the supernatant was discarded after centrifugation to obtain substance B.
[0028] 3) Ad...
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[0030] Example 2
[0031] The present invention provides a method for extracting crude solanesol by microwave enzymatic method using potato leaves as raw materials, which comprises the following steps:
[0032] 1) Crush dried potato leaves, pass a 60-mesh sieve, add distilled water, accurately weigh 50 grams of potato leaves, add 750ml of distilled water, ultrasonically wash for 40 minutes under the conditions of ultrasonic power 200W, frequency 40KHz, and temperature 50°C, and then filter. Obtain solid substance A;
[0033] 2) To substance A, add 1000ml of cellulase solution with a mass concentration of 0.0005g / ml prepared with a citric acid buffer with a pH of 5.2, at a microwave power of 150W, a frequency of 2450MHz, a temperature of 40℃, and a stirring speed of 110r / min Under the conditions, microwave-assisted enzymatic hydrolysis was performed for 40 minutes, and centrifuged at 3800 rpm for 12 minutes. After centrifugation, the supernatant was discarded to obtain substance B.
...
Example Embodiment
[0036] Example 3
[0037] The present invention provides a method for extracting crude solanesol by microwave enzymatic method using potato leaves as raw materials, which comprises the following steps:
[0038] 1) Crush dried potato leaves, pass a 50-mesh sieve, add distilled water, accurately weigh 60 grams of potato leaves, add 600ml of distilled water, ultrasonically wash for 20 minutes under the conditions of ultrasonic power 150W, frequency 40KHz, and temperature 40℃, and then filter. A solid substance was obtained.
[0039] 2) 600ml of cellulase solution with a mass concentration of 0.004g / ml is prepared by adding citrate buffer solution with pH 5.6 to substance A, under the conditions of microwave power of 250W, frequency of 2450MHz, temperature of 60℃ and stirring speed of 100r / min Under the microwave-assisted enzymatic hydrolysis for 60 minutes, centrifuge for 15 minutes at 3600 rpm, and discard the supernatant after centrifugation to obtain substance B.
[0040] 3 Add 1500m...
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