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Microwave enzyme method-assisted method for extracting crude solanesol from potato leaf

An auxiliary extraction and potato technology, which is applied in the separation/purification of hydroxyl compounds, organic chemistry, fermentation, etc., can solve the problems that lye is easy to cause environmental pollution, etc., and achieve significant economic benefits and less waste liquid discharge.

Inactive Publication Date: 2011-02-23
NORTHWEST NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantage of the above process is that solanesol will be partially degraded or isomerized when it is heated in an alkaline environment for a long time, and the lye produced after saponification is likely to cause environmental pollution. In view of this, microwave enzymatic assisted extraction of solanesol The research on solanesol not only protects the free solanesol from being degraded or isomerized by strong alkali, but also releases the bound solanesol as much as possible
So far, domestic and foreign literature and patents have not involved the research on the extraction of solanesol by microwave enzymatic method using potato leaf waste as raw material

Method used

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  • Microwave enzyme method-assisted method for extracting crude solanesol from potato leaf
  • Microwave enzyme method-assisted method for extracting crude solanesol from potato leaf
  • Microwave enzyme method-assisted method for extracting crude solanesol from potato leaf

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Effect test

Embodiment 1

[0025] The invention provides a method for extracting crude solanesol by using potato leaves as a raw material and aided by microwave enzymatic method, comprising the following steps:

[0026] 1) Crush dried potato leaves, pass through a 40-mesh sieve, add distilled water, accurately weigh 40 grams of potato leaves, add 500ml of distilled water, and ultrasonically wash with water for 60 minutes under the conditions of ultrasonic power 250W, frequency 40KHz, and temperature 60°C, and then filter. Get substance A;

[0027] 2) Add 800ml of cellulase solution with a mass concentration of 0.002g / ml prepared with a citric acid buffer solution with a pH of 4.4 to substance A, and use a microwave with a power of 50W, a frequency of 2450MHz, a temperature of 30°C, and a stirring speed of 120r / min. Under the conditions, microwave assisted enzymatic hydrolysis for 15 minutes, centrifuged at 4000 rpm for 10 minutes, discarded the supernatant after centrifugation, and obtained substance B....

Embodiment 2

[0031] The invention provides a method for extracting crude solanesol by using potato leaves as a raw material and aided by microwave enzymatic method, comprising the following steps:

[0032] 1) Dried potato leaves were crushed, passed through a 60-mesh sieve, added distilled water, accurately weighed 50 grams of potato leaves, added 750ml of distilled water, ultrasonically washed with water for 40 minutes under the conditions of ultrasonic power 200W, frequency 40KHz, and temperature 50°C, and then filtered. Obtain solid substance A;

[0033] 2) Add 1000ml of cellulase solution with a mass concentration of 0.0005g / ml prepared with a citric acid buffer solution with a pH of 5.2 to substance A, and use a microwave with a power of 150W, a frequency of 2450MHz, a temperature of 40°C, and a stirring speed of 110r / min Under the conditions, microwave assisted enzymatic hydrolysis for 40 minutes, centrifuged at 3800 rpm for 12 minutes, discarded the supernatant after centrifugation,...

Embodiment 3

[0037] The invention provides a method for extracting crude solanesol by using potato leaves as a raw material and aided by microwave enzymatic method, comprising the following steps:

[0038] 1) Crush dried potato leaves, pass through a 50-mesh sieve, add distilled water, accurately weigh 60 grams of potato leaves, add 600ml of distilled water, and ultrasonically wash for 20 minutes under the conditions of ultrasonic power 150W, frequency 40KHz, and temperature 40°C, and then filter. A solid substance was obtained.

[0039] 2) Add 600ml of cellulase solution with a mass concentration of 0.004g / ml prepared by citric acid buffer solution with a pH of 5.6 to substance A, under the conditions of a microwave power of 250W, a frequency of 2450MHz, a temperature of 60°C, and a stirring speed of 100r / min Microwave assisted enzymolysis for 60 minutes, centrifuged at 3600 rpm for 15 minutes, discarded the supernatant after centrifugation, and obtained substance B.

[0040] 3. Add 1500...

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Abstract

The invention discloses a microwave enzyme method-assisted method for extracting crude solanesol from a potato leaf. The method comprises the following steps of: changing the permeability of the cell wall of the potato leaf serving as a raw material by using microwave-assisted cellulase; and hydrolyzing combined-state solanesol by using lipase. The defect of reduction in solanesol yield caused by inevitable degradation or isomerization of solanesol during alkaline saponification of the conventional process is overcome.

Description

technical field [0001] The invention relates to a method for extracting crude solanesol from potato stems and leaves by using potato leaves as raw materials and using microwave enzymatic method to assist. Background technique [0002] Solanesol is a long-chain semiterpene alcohol compound, belonging to tetrasesquiterpene alcohols. Pure solanesol is white or light yellow waxy solid, easily soluble in organic solvents such as n-hexane, acetone, petroleum ether, etc. Insoluble in water. The structure of solanesol contains multiple non-conjugated double bonds, which has a very strong ability to absorb free radicals and plays a very important role in biochemistry. It not only has antibacterial, anti-inflammatory, cardiovascular disease and anti-ulcer effects, And it is an important pharmaceutical intermediate, mainly used in the synthesis of coenzyme Q10, vitamin K, anticancer synergist SDB, etc. [0003] Solanesol widely exists in higher plants in free and combined forms, and ...

Claims

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Application Information

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IPC IPC(8): C07C29/76C12P7/04C07C33/02
Inventor 张继王自社赵保堂姚健马君义梁俊玉王惠明高莹王莉郭肖付丽娟彭小凤薛文君
Owner NORTHWEST NORMAL UNIVERSITY
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