Food waste degradation and elimination type microbial bacterial agent, preparation method thereof and bacteria used thereby

A technology for microbial inoculants and food waste, applied in the directions of microorganism-based methods, microorganisms, microorganisms, etc., can solve the problems of reducing solid waste discharge, increasing sewage treatment pressure, and environmental pollution of kitchen food waste, and achieving harmless effect of reduction and high reduction

Active Publication Date: 2011-04-13
ZHEJIANG UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

This technology increases the pressure of sewage treatment while reducing the discharge of solid wa...

Method used

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  • Food waste degradation and elimination type microbial bacterial agent, preparation method thereof and bacteria used thereby
  • Food waste degradation and elimination type microbial bacterial agent, preparation method thereof and bacteria used thereby
  • Food waste degradation and elimination type microbial bacterial agent, preparation method thereof and bacteria used thereby

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1, collection, isolation, screening and identification of Bacillus cereus (Bacilus cereus)

[0037] 1), collection, separation, purification

[0038] Take 10g of organic fertilizer obtained from high-temperature composting of livestock and poultry manure, put it in a 250mL triangular flask filled with 100mL beef extract peptone liquid medium, shake at 100r / min for 24h enrichment culture, dip the obtained suspension into the bacteria Streak on the selective medium plate, culture at 30°C for 48 hours, pick several single colonies with different shapes from the plate, respectively streak on the bacterial selective medium plate, repeat several times until each colony is purified, Several bacterial isolates were obtained.

[0039] Bacterial selective medium plate preparation: beef extract 3g, peptone 10g, NaCl 5g, agar 18g, add distilled water to 1000mL, adjust pH to 7.0-7.2; 2 , sterilized at 121°C for 20 minutes. After cooling, add 30 units / L of nystatin and 200...

Embodiment 2

[0073] Embodiment 2: Composition of food waste degrading and eliminating type microbial bacterial agent III

[0074] Total bacteria count: 2.8×10 10 cfu / mL

[0075] Actinomucor elegans No.1: 1.3×10 10 cfu / mL

[0076] Bacillus cereus No.6: 1.5×10 10 cfu / mL

Embodiment 3

[0077] Embodiment 3: Composition of food waste degrading and eliminating type microbial bacterial agent III

[0078] Total bacteria count: 2.5×10 11 cfu / mL

[0079] Actinomucor elegans No.1: 1.4×10 11 cfu / mL

[0080] Bacillus cereus No.6: 1.1×10 11 cfu / mL

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PUM

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Abstract

The invention discloses Bacilus cereus of which the collection name is No.6, the collection unit is General Microbiology Center for China Committee for Culture Collection of Microorganisms, the collection address is Institute of Microbiology of Chinese Academy of Sciences, No.3, N0.1 yard, Beichen west road, Chaoyang district, Beijing city, the collection date is June 1st, 2010, and the collection number is CGMCCNO.3884. The invention also discloses a food waste degradation and elimination type microbial bacterial agent III, which is a liquid composite bacterial agent of actinomucor elegans with the microbial content of 3.0*10<8> to 1.4*10<11>cfu/ml and the Bacilus cereus with the microbial content of 3.0*10<8> to 1.8*10<11>cfu/ml. The invention also discloses a preparation method of the food waste degradation and elimination type microbial bacterial agent III. The microbial bacterial agent III is used for degrading food waste.

Description

technical field [0001] The invention belongs to the field of domestic waste treatment, and relates to a microbial degradation and elimination technology for food waste, in particular to a preparation method of a microbial antibacterial agent for food waste. Background technique [0002] With the rapid development of our country's social economy, not only the amount of domestic waste of urban residents is increasing, but also the proportion of food waste such as melon peels and shells, leftovers and leftovers in domestic waste is also increasing. Due to the high moisture content (90%) and low incineration calorific value (2100-3100kJ / kg) of food waste, after being mixed with other urban waste components, landfill disposal will not only occupy valuable land resources but also produce a large amount of leachate and pollute groundwater. system; the use of incineration for power generation disposal cannot meet the calorific value requirements of garbage (ie above 5000kJ / kg), whic...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/00C12N1/14B09B3/00C12R1/085C12R1/645
Inventor 方萍吴海龙张允升张杰锋
Owner ZHEJIANG UNIV
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