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Ultrahigh pressure liquid phase detection method for prepared Chinese herb medicine

An ultra-high pressure liquid phase and detection method technology, applied in the ultra-high field, can solve the problems of difficult separation and long total time.

Inactive Publication Date: 2013-07-03
NAT INST FOR FOOD & DRUG CONTROL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the total time required to establish fingerprints by using HPLC technology is generally long, and the separation degree is difficult to achieve an ideal situation.

Method used

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  • Ultrahigh pressure liquid phase detection method for prepared Chinese herb medicine
  • Ultrahigh pressure liquid phase detection method for prepared Chinese herb medicine
  • Ultrahigh pressure liquid phase detection method for prepared Chinese herb medicine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0140] Example 1. The quality detection method of Tongren Wuji Baifeng Pills (Darmi Pills, Tongrentang Pharmaceutical)

[0141] Instruments and reagents: Waters Acquity UPLC TM Ultra-high performance liquid chromatography-diode array detector (PDA), Waters Empower 2 data processing software system.

[0142] BüCHI rotary evaporator (Rotavapor R-114; Waterbath B-480); Lion Ding SHB-III circulating water multi-purpose vacuum pump (Zhengzhou Changcheng Technology Industry and Trade Co., Ltd.); KQ-250DE medical numerical control ultrasonic cleaner (Kunshan Ultrasonic Instrument Co., Ltd.); FW100 high-speed universal pulverizer (Tianjin Test Instrument Co., Ltd.). D-101 macroporous resin (Anhui Sanxing Resin Technology Co., Ltd.).

[0143] Methanol and ethanol are analytically pure reagents; UPLC uses acetonitrile (J.T.Baker Company) as chromatographically pure. Water is pure water, passed through Millipore 0.22μm filter membrane.

[0144] Paeoniflorin, Liquiritin, Ginsenoside R...

Embodiment 2

[0155] Example 2. The detection method of active ingredients in Wuji Baifeng Pills (Shuiwan, Huiren Pharmaceutical)

[0156] 1. Preparation of the solution of the test sample: Accurately weigh Wuji Baifeng Pills (water pill 6g), add methanol equivalent to about 8 times the amount of the test sample, dissolve, preferably ultrasonically treat for 20-40 minutes, filter, and recover the filtrate Concentrate the solvent to dryness, add about 50mL of water to the residue to dissolve, pass through a D101 macroporous resin adsorption column (the resin packed in the column is about 60g, the inner diameter is 2cm, and the length is 12cm), elute with about 300mL of water, discard the water, and then Elute with 50mL of ethanol with 25-35%wt, discard the eluate, continue to elute with 200mL of ethanol with 80%wt, collect the eluate, evaporate to dryness, dissolve the residue with methanol and transfer it to a 5mL volumetric flask, Add methanol to the scale, pass through a microporous membr...

Embodiment 3

[0161] Example 3. Detection method of active ingredients in other traditional Chinese medicine preparations containing two or more medicinal ingredients from Radix Paeoniae Alba, Ginseng, Danshen, Artemisia annua, Licorice, Rhizoma Chuanxiong, Angelica, Radix Astragali, and Rhizoma Cyperi

[0162] 1. Preparation of the test product solution: Accurately weigh 6 g of the test product (the test product contains Radix Paeoniae Alba, Radix Ginseng, Salvia miltiorrhiza), add methanol equivalent to about 8 times the amount of the test product, dissolve, filter, and the filtrate reclaims the solvent Concentrate to dryness, add about 50mL water to the residue to dissolve, pass through a D101 type macroporous resin adsorption column (about 60g of resin packed in the column), elute with about 300mL water, discard the water, and use 50mL ethanol with 25-35%wt Elution, discard the eluate, elute with 75% wt about 200mL ethanol, collect the eluate, evaporate to dryness, dissolve the residue w...

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Abstract

The invention provides an ultrahigh pressure liquid phase detection method for a Chinese patent medicine containing at least two medicinal materials of white paeony root, ginseng, root of red-rooted salvia, sweet worm wood herb, liquoric root, szechuan lovage rhizome, Chinese angelica, astragalus and nutgrass galingale rhizome. The method comprises the following steps of: preparing solution of a test sample and solution of a contrast medicinal material; performing ultra performance liquid chromatography (UPLC) detection by taking acetonitrile-water as a mobile phase and using an ultraviolet detector and / or a mass spectrum detector, wherein the detection wavelength is between 201 and 320nm; and performing result detection, namely detecting whether a corresponding chromatographic peak exists or not at a position where the retention time is as same as that of a contrast medicinal material chromatogram in a chromatogram of the test sample. Compared with thin layer identification and high performance liquid chromatography (HPLC) detection methods, the ultrahigh pressure liquid phase detection method successfully improves the resolution of the chromatogram, shortens the analysis cycle, and ensures more precise and stable results.

Description

technical field [0001] The invention provides an ultra-high pressure liquid phase detection method of a compound Chinese patent medicine, which is a method for detecting active ingredients in a compound Chinese patent medicine (also known as a large prescription) preparation by using an ultra-high pressure liquid phase, especially a method containing Radix Paeoniae Alba, Ginseng, UHPLC detection method for active ingredients in Chinese patent medicines containing two or more ingredients in Danshen, Artemisia annua, Licorice, Chuanxiong, Angelica, Astragalus, and Rhizoma Cyperi. Background technique [0002] Traditional Chinese medicine compound is the main form and method of clinical medicine in traditional Chinese medicine. Each medicine in the prescription contains a variety of chemical components. These inherent chemical components are the material basis for the efficacy of the traditional Chinese medicine compound. Reflecting the overall curative effect embodied by tradi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/36A61K36/8905A61K36/71A61K36/537A61K36/484A61K36/481A61K36/258A61K36/236
Inventor 林瑞超王钢力聂黎行陈佳李志猛
Owner NAT INST FOR FOOD & DRUG CONTROL
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