Chitosan nano-biological preparation, and preparation method and application thereof

A technology of chitosan nano and biological preparations, applied in botany equipment and methods, chemicals for biological control, biocides, etc., can solve the problems of chitosan not easy to dissolve, high viscosity, large particles, etc.

Inactive Publication Date: 2011-05-04
LIAONING NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0010] Because general chitosan is not easy to dissolve, has high viscosity and large particles, it is not easy to be directly absorbed by plants, so chitin has encountered a bottleneck in the application of plant growth regulation.

Method used

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  • Chitosan nano-biological preparation, and preparation method and application thereof
  • Chitosan nano-biological preparation, and preparation method and application thereof
  • Chitosan nano-biological preparation, and preparation method and application thereof

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Embodiment Construction

[0025] Dissolve chitin in 1vt% acetic acid solution so that the concentration of chitin is 2mg / ml, adjust the pH value to 4.6-4.7 with 10mol / L sodium hydroxide, and degrade for 4-8 hours to obtain chitin acetic acid solution. In the solution, add 1 mg / mL sodium tripolyphosphate solution dropwise, the mass ratio of chitin acetic acid solution and sodium tripolyphosphate solution is 5:1, stir magnetically for 30 minutes, and pulse ultrasonic for 15 minutes under the condition of ice bath, the condition of pulse ultrasonic is power 85%, every 2 seconds for 3 seconds to obtain cross-linked and uniformly dispersed chitosan nano-biological preparations.

[0026] UV spectrophotometer to measure the stability of chitosan nano-microspheres:

[0027] The stability of nano-chitosan was determined by turbidimetric method. The cross-linked suspension was left at room temperature at 20°C for 3d, 7d, 14d, 20d, and 30d respectively, and the change in absorbance was measured with an ultraviol...

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Abstract

The invention discloses a chitosan nano-biological preparation, and a preparation method and application thereof. The preparation method comprises the following steps of: dissolving chitosan in 1 volume percent acetic acid solution to keep the concentration of the chitosan at 2mg / ml; adjusting pH value to be between 4.6 and 4.7 by using 10mol / L sodium hydroxide; degrading for 4 to 8 hours to obtain chitosan acetic acid solution; dropwise adding 1mg / mL of sodium tripolyphosphate solution into the chitosan acetic acid solution, wherein the mass ratio of the chitosan acetic acid solution to the sodium tripolyphosphate solution is 5 to 1; magnetically stirring for 30 minutes; performing pulse ultrasonic processing for 15 minutes in an ice bath by using 85 percent of power; and opening the ultrasonic sound for 3 seconds every 2 seconds to prepare the cross-linked and uniformly dispersed chitosan nano-biological preparation. Nano-chitosan is used as a novel plant growth adjusting substance which can induce a plant to secrete resistant enzyme, excite expression of a resistant gene and effectively improve the resistance of the plant against adverse environmental stress.

Description

technical field [0001] The invention relates to chitosan nano biological preparation and its preparation. Background technique [0002] Chitosan is one of the most important derivatives of chitin after deacetylation. Chitin, also known as chitin and chitin, is an amino polysaccharide polymer with a chemical name of β-(1,4)-2-acetylamino-2-deoxy-D-glucose, and its molecular formula is (C 8 h 15 NO 6 ) n , with a molecular weight of about one million. Chitin is the only positively charged natural biopolymer material that exists in nature. It is widely found in the shells of lower animals such as crabs and shrimps, the carapaces of insects such as crickets, and shells of oysters, as well as some lower plants such as The cell walls of algae and fungi are rich in resources. They are the second largest natural polymer compound after cellulose in the world, and they are an inexhaustible renewable resource. [0003] The molecular structure of chitin is as follows: [0004] ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N43/16A01P21/00
Inventor 吕建洲马晓旭孙微刘照斌刘美静张勇
Owner LIAONING NORMAL UNIVERSITY
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