Shake flask fermentation method for preparing calvatia gigantean liquid fermentation culture medium

A large bald puffball, shake flask fermentation technology, applied in the direction of botanical equipment and methods, application, fertilizer mixture, etc., to achieve high yield, simple process equipment, and short cycle

Inactive Publication Date: 2011-06-08
武侯区巅峰机电科技研发中心
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, artificial cultivation has not been successful, but mycelium can be used for submerged fermentation culture

Method used

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  • Shake flask fermentation method for preparing calvatia gigantean liquid fermentation culture medium
  • Shake flask fermentation method for preparing calvatia gigantean liquid fermentation culture medium
  • Shake flask fermentation method for preparing calvatia gigantean liquid fermentation culture medium

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Embodiment Construction

[0015] The method for preparing the big bald puffball liquid fermentation medium by using the shaking flask fermentation method, the culture time is 1-4 days, the temperature is 20-28 DEG C, and the initial pH is 5.0-6.0.

[0016] Preferably, the culture time is 2 days, the culture temperature is 22° C., and the initial pH is 5.5.

[0017] Strain activation medium - PDA medium: 200g potato, 20g glucose, 20g agar, 1000mL water.

[0018] Preparation method: Weigh 200g of peeled potatoes, cut into pieces, add water to boil, filter with two layers of gauze after 30min, add 20g of glucose to the filtrate, add water to 1000ml, boil, subpackage, and sterilize at 121°C for 30min. Remove to cool and set aside.

[0019] Shake flask fermentation medium - PDA liquid medium: 200g of potatoes, 20g of glucose, 1000mL of water.

[0020] On the ultra-clean workbench, take an activated slant culture of the bald puffball, first add 10ml of water to the test tube, and gently scrape off the bact...

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Abstract

The invention relates to a shake flask fermentation method for preparing a calvatia gigantean liquid fermentation culture medium, which is characterized in that: the culture time is 1 to 4 days, the temperature is between 20 and 28 DEG C, and the initial pH value is 5.0 to 6.0. The calvatia gigantean liquid fermentation culture medium is obtained by controlling the initial pH value and the fermentation time and temperature, and the prepared culture medium can culture massive mycelia and fermentation products thereof in a short time.

Description

technical field [0001] The invention relates to a method for preparing a large bald puffball liquid fermentation medium by using a shake flask fermentation method. Background technique [0002] Big bald puffball, also known as big puffball, puffball, flattery bag, is a kind of edible and medicinal fungus, which has considerable economic value. The fruiting body is large, nearly spherical to spherical, 15-36cm in diameter, or larger, without sterile base or very small, connected to the ground by thick fungal cords, coated with white, and then becomes dirty white, with a waxy outer coating and thicker It is composed of inner capsule, which is slightly fluffy at the beginning, gradually becomes smooth and brittle, and cracks and falls off in pieces after maturity, exposing light blue and brown spores. It grows on grasslands in the wilderness in summer and autumn, solitary to in groups, and can form "mushroom circles" in the mountainous grasslands of Xinjiang and the Humeng gra...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/04C05G1/00
Inventor 黄友华
Owner 武侯区巅峰机电科技研发中心
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