Two-channel detection card for simultaneously detecting glibenclamide and gliclazide and detection method thereof

The technology of glyburide and detection card is applied in the field of detection of illegally added western medicine components in traditional Chinese medicine, which can solve the problems of complicated operation and high professional requirements, and achieve the effects of high sensitivity, rapid detection and easy preparation.

Inactive Publication Date: 2011-06-08
无锡安迪生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a device capable of simultaneously detecting glibenclamide and gliclazide, which overcomes the defects of cum

Method used

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  • Two-channel detection card for simultaneously detecting glibenclamide and gliclazide and detection method thereof
  • Two-channel detection card for simultaneously detecting glibenclamide and gliclazide and detection method thereof
  • Two-channel detection card for simultaneously detecting glibenclamide and gliclazide and detection method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0023] A dual-channel detection card for glibenclamide and gliclazide, which is provided with a test strip 2 in the detection card casing 1, and the structure of the detection card casing 1 and the setting of the test strip 2 refer to the attached figure 1 And attached figure 2 , The detection card shell 1 is a long flat shell-shaped shell with a length of 7cm, a width of 2cm, a thickness of 0.5cm, and a wall thickness of the thin shell of 0.1cm, made of engineering plastics. The detection card casing 1 is composed of two halves of a casing cover and a casing seat, and a detection window 3 and a sample injection hole 4 are arranged on the casing cover of the detection card casing 1 . The test strip 2 is placed in the shell seat of the test card shell 1 .

[0024] The test strip 2 is a thin strip with a multilayer structure, the length of the strip is about 6.0cm, the width of the strip is about 0.4cm, and the thickness is less than 2.5mm. The test strip 2 has a multilayer s...

Embodiment 2

[0027] The test samples are capsules, tablets, pills and other hypoglycemic traditional Chinese medicine samples, and the processing methods are as follows:

[0028] (1) Randomly collect a certain amount of samples to be tested and crush them evenly;

[0029] (2) Weigh 5g of the pulverized sample, add 10mL of sample extract, stir and mix for more than 5 minutes, and shake for 30 minutes;

[0030] The extract solution is configured as follows: add 30mL distilled water to 70mL methanol;

[0031] (3) Transfer the solution obtained in step (2) into a 10mL centrifuge tube, centrifuge at 4000r / min for 10min, and take the supernatant after centrifugation; if the supernatant is clear, use the ratio of supernatant: sample diluent as 1:3 To dilute, take 100 μL of the supernatant and add 300 μL of sample diluent, mix well and then measure; Measured after mixing;

[0032] The sample diluent is pH7.6, 10mmoL PBST solution;

[0033] The main reaction of the test card is the immunologica...

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Abstract

The invention relates to a two-channel detection card for simultaneously detecting glibenclamide and gliclazide and a detection method thereof, belonging to the technical field of detection of western medicine illegally added into Chinese medicine. The surface of a detection card casing is provided with a detection window opening and a sampling hole, a test strip is arranged in the casing, a nitrocellulose membrane is attached to the middle of a bearing back panel, a water sucking membrane is attached to one end of the bearing back panel, a sample pad is attached to the other end of the bearing back panel, two colloidal gold membranes are clamped and attached between the nitrocellulose membrane and the sample pad, wherein the colloidal gold membranes are glass fiber membranes which respectively contain an anti-glibenclamide colloidal gold labeled monoclonal antibody and anti-gliclazide colloidal gold labeled monoclonal antibody, three print display bands are intervally arranged on the nitrocellulose membrane along the traverse direction, one is a detection band containing a glibenclamide protein conjugate, another one is a detection band containing a gliclazide protein conjugate, and the third one is a quality control band containing an anti-rabbit antibody or an anti-mouse antibody, the sample pad is arranged just opposite to the sampling hole, and the nitrocellulose membrane is arranged just opposite to the detection window opening. The detection card can be used for simultaneously detecting two western medicine components illegally added into a glucose-lowering Chinese medicine sample, has the advantages of detection cost saving, rapid detection, high sensitivity, accurate result, and convenience for use.

Description

technical field [0001] The invention relates to a device for detecting illegally added western medicine components in hypoglycemic traditional Chinese medicine, in particular to a dual-channel detection card for simultaneously detecting glibenclamide and gliclazide, and belongs to the technical field of detection of illegally added western medicine components in traditional Chinese medicine. Background technique [0002] Both glibenclamide and gliclazide are sulfonylurea hypoglycemic drugs, which mainly play a role by promoting insulin secretion. In addition, they can also strengthen the binding of insulin to receptors, relieve the effect of insulin resistance on receptors, and make insulin The effect is strengthened. It has definite curative effect and low price, and is widely used in clinical practice, but it also has certain toxic and side effects. Glibenclamide can cause hypoglycemia and a series of other serious adverse reactions such as adrenal insufficiency and terat...

Claims

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Application Information

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IPC IPC(8): G01N33/558
Inventor 周坚熊晓芳吴文晔
Owner 无锡安迪生物工程有限公司
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