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Macro fungi insecticide for preventing and controlling aromia bungii

A fungal pesticide and a large-scale technology, which is applied in the field of microbial source large-scale fungal pesticides and their preparation, can solve the problems of short drug effect time, low lethality, strong pertinence, etc. Good control effect and long-lasting effect

Inactive Publication Date: 2011-06-15
SHENYANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This insecticide has the advantages of being easy to degrade, has no drug residue, does not kill natural enemies of insects, and does not produce resistance to insecticides, but has the following disadvantages: 1. The lethality rate is not high, and the mortality rate of larvae is low, only 22.6%; 2. It has poor poisoning selectivity to the larvae of the pink-necked longhorn beetle, but is highly targeted to cabbage caterpillar and pine caterpillar; 3. The drug effect time is short, and it is not resistant to high temperature. When the temperature is higher than 28 ℃, the spores will be inactivated, and the use effect in high temperature season not good
The application of macrofungal insecticides in the control of P. chinensis has not been found so far

Method used

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  • Macro fungi insecticide for preventing and controlling aromia bungii

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] A macrofungal insecticide for preventing and controlling the pink-necked longhorn beetle. Its components and contents are: 40% crude pedopeptide toxinoids of P. Purpose fungus chaff powder.

[0046] A macrofungal insecticide for preventing and treating the pink-necked longhorn beetle, the preparation method of which is:

[0047] 1. Screening of macrofungi

[0048] One test tube each of the parent species of Amanita fruticosa and Amanita striata containing toxic peptide toxins was screened from large fungi.

[0049] 2. Shaker liquid submerged fermentation

[0050] (1), liquid medium: 200 grams of potatoes, 20 grams of glucose, NaNO 3 3 grams, KH 2 PO 4 1g, KCl0.5g, MgSO 4 ·7H 2 O 0.05 g, FeSO 4 0.01g, 1000ml distilled water, PH7.0.

[0051] (2) Take two triangular flasks with a capacity of 1000 ml, label them as bottle A and bottle B, and then add 500 ml of liquid medium to bottle A and bottle B respectively. Sterilize at 121°C and 0.1 MPa for 15 minutes.

[0...

Embodiment 2

[0063] A macrofungal insecticide for preventing and controlling the pink-necked longhorn beetle. Its components and contents are: 40% crude pedopeptide toxinoids of P. Purpose fungus chaff powder.

[0064] A macrofungal insecticide for preventing and treating the pink-necked longhorn beetle, the preparation method of which is:

[0065] 1. Screening of macrofungi

[0066] One test tube each of the parent species of Amanita fruticosa and Amanita striata containing toxic peptide toxins was screened from large fungi.

[0067] 2. Shaker liquid submerged fermentation

[0068] (1), liquid medium: 200 grams of potatoes, 20 grams of glucose, NaNO 3 3 grams, KH 2 PO 4 1g, KCl0.5g, MgSO 4 ·7H 2 O0.05 g, FeSO 4 0.01g, 1000ml distilled water, PH7.0.

[0069] (2) Take two triangular flasks with a capacity of 500 ml, label them, and mark them as bottle A and bottle B, and then add 200 ml of liquid culture medium to bottle A and bottle B respectively. Sterilize at 126°C, 0.1 MPa for...

Embodiment 3

[0081] A macrofungal insecticide for preventing and controlling the pink-necked longhorn beetle. Its components and contents are: 40% crude pedopeptide toxinoids of P. Purpose fungus chaff powder.

[0082] A macrofungal insecticide for preventing and treating the pink-necked longhorn beetle, the preparation method of which is:

[0083] 1. Screening of macrofungi

[0084] One test tube each of the parent species of Amanita fruticosa and Amanita striata containing toxic peptide toxins was screened from large fungi.

[0085] 2. Shaker liquid submerged fermentation

[0086] (1), liquid medium: 200 grams of potatoes, 20 grams of glucose, NaNO 3 3 grams, KH 2 PO 4 1g, KCl0.5g, MgSO 4 ·7H 2 O 0.05 g, FeSO 4 0.01g, 1000ml distilled water, PH7.0.

[0087] (2) Take two triangular flasks with a capacity of 1000 ml, label them as bottle A and bottle B, and then add 600 ml of liquid medium to bottle A and bottle B respectively. Sterilize at 123°C, 0.1 MPa for 18 minutes.

[0088...

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PUM

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Abstract

The invention discloses a macro fungi insecticide for preventing and controlling aromia bungii, which contains the following components and the contents thereof: 40% of crude product of lepiota helveola phallotoxin toxins, 35% of crude product of amanita phalloides phallotoxin toxins and 25% of 10-mesh fungus chaff powder. The preparation method comprises the steps of: 1, screening of macro fungi; 2, liquid deep fermentation on a shaking table; 3, ultrasonication and refrigerated centrifugation of fermentation liquid; 4, extraction of crude products of phallotoxin toxins; 5, selection and treatment of a carrier; and 6, formation. The macro fungi insecticide for preventing and controlling aromia bungii has the characteristics of high lethality, strong selectivity, long effective time and high temperature resistance.

Description

technical field [0001] The invention relates to a biological pesticide, in particular to a microbial-source macrofungal insecticide for preventing and controlling garden borer pests, the longhorn beetle, and a preparation method thereof. Background technique [0002] Peach-necked longhorn beetle is a kind of very serious dry borer pest. Its harm is mainly that the larvae eat stone fruit trunks such as peach trees, apricot trees and cherries, form curved tunnels in the xylem, destroy the phloem and xylem, and cause bark damage. Falling off, weakening of tree vigor, and easy breaking of branches often cause the whole tree to die. It is recognized as the most difficult pest to control. [0003] At present, the microbial source fungal insecticides used to control the pink-necked beetle are mainly Beauveria bassiana insecticides. This insecticide is a live bacterial preparation made of Beauveria bassiana spore powder. After the spores invade the larvae of the beetle, a large num...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N65/00A01P7/04
Inventor 杨绍斌韩俊艳王军
Owner SHENYANG UNIV
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