Method for determining alkaloid in coffee tincture

A determination method and technology of coffee tincture, applied in the direction of measuring devices, instruments, scientific instruments, etc., to achieve the effect of simple pretreatment, good repeatability and recovery rate

Active Publication Date: 2011-06-15
ZHENGZHOU TOBACCO RES INST OF CNTC
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At present, the simultaneous determination of these th

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  • Method for determining alkaloid in coffee tincture
  • Method for determining alkaloid in coffee tincture
  • Method for determining alkaloid in coffee tincture

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The present invention will be further explained through implementation examples below:

Chromatographic conditions: Agilent1200 liquid chromatography, equipped with diode array detector, detection wavelength 265nm. Chromatographic column: watersC18 analytical column (250mm×4.6mm, 5μm). Mobile phase: 0.1% formic acid water (A), methanol (B); gradient elution, starting with 2% B, 10% B after 5 min, 50% B after 12 min, 100% B after 15 min, hold for 5 min, 2% for 21 min B equilibrate the column for 4 min. The flow rate is 1mL / min. The analysis time is 25 minutes in total. The column temperature is 30°C.

1) Formulation of standard working curve:

The standard curve of fenugreek base, nicotine acid and caffeine was prepared with methanol, and the concentrations of the mother liquor were 1.7 mg / mL, 13.3 mg / mL and 11.0 mg / mL, respectively. The standard curve range of fenugreekine is 4.25×10-4-0.17mg / mL, the standard curve equation is y=20234x+0.0806, R2=1; the standard curve range ...

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Abstract

The invention relates to a method for determining alkaloid in coffee tincture. The method is characterized in that water is taken as sample diluting solvent, mixed solution of methyl alcohol and 0.1% methanoic acid aqueous solution is taken as a mobile phase, and the high performance liquid chromatography is adopted to simultaneously detect caffeine, trigonelline and nicotinic acid in coffee tincture, wherein a diode array detector is adopted. The method has the key points that the water is taken as the sample diluting solvent and the 0.1% methanoic acid aqueous solution is taken as the mobile phase. The water is taken as the sample diluting solvent, thus pigment in a sample which enters into a chromatographic column is reduced, a chromatographic column is effectively protected, a sample preprocessing solvent is environmentally friendly, and the chromatographic peak shape of alkaloid is not influenced. Compared with pure water taken as the mobile phase, the 0.1% methanoic acid aqueoussolution is taken as the mobile phase, and the chromatographic peak shape can be effectively improved, thus being beneficial to accurate determination of nicotinic acid. The method has the advantagesof simple preprocessing, good repeatability and high recovery rate and can be applicable to analysis of samples on a large scale.

Description

A method for the determination of alkaloids in coffee tincture technical field The invention relates to a method for detecting alkaloids, in particular to a method for detecting alkaloids in coffee tincture, which can simultaneously detect three kinds of alkaloids in coffee tincture: caffeine, trigonelline and nicotine acid. Background technique Coffee tincture is obtained by extracting roasted coffee seeds with a certain concentration of ethanol. The product contains a variety of alkaloids and other heterocyclic compounds, as well as furfuryl alcohol, sulfur derivatives and a small amount of volatile acids. It has typical sensory characteristics such as aroma and bitter aroma, and it can enhance the aroma, increase the roasted aroma, and make the taste bitter when added to cigarettes. The main alkaloid in coffee beans is caffeine. The content in green coffee beans varies with different varieties. It has a distinct bitter taste and has significant physiological effects o...

Claims

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Application Information

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IPC IPC(8): G01N30/02
Inventor 丁丽谢复炜夏巧玲王洪波郭军伟张晓兵
Owner ZHENGZHOU TOBACCO RES INST OF CNTC
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