Method for determining content of azodicarbonamide in leather by HPLC (high performance liquid chromatography)

Abstract

The invention discloses a method for determining the content of azodicarbonamide in the leather by HPLC (high performance liquid chromatography). The method comprises the following steps: step 1, preparing three samples; step 2, performing HPLC determination on the three solutions prepared in step 1 under the condition of HPLC. With adoption of the method, detection of the content of azodicarbonamide in leather can be well controlled, health and safety of consumers are guaranteed, pretreatment is simple, and the method can be used for accurately detecting the content of azodicarbonamide in theleather.

Description

technical field [0001] The invention relates to a method for determining the content of azodicarbonamide in leather by high performance liquid chromatography. Background technique [0002] Azodicarbonamide, referred to as AC foaming agent or ADC foaming agent, is suitable for a variety of resins, such as PVC, PE, EVA, EPDM, PS, PU, ​​epoxy resin, natural and synthetic rubber, etc., composed of hydrazine hydrate; urea Condensate with sulfuric acid to form the intermediate biurea, and then oxidize to obtain the finished product. The raw material hydrazine hydrate is highly corrosive and highly toxic. It can attack glass, rubber, leather and cork, etc. Vapor and liquid can corrode the skin, eyes, nose, throat and mucous membranes, etc. There is sufficient evidence that azodicarbonamide can induce asthma and other respiratory symptoms. On December 19, 2012, it was included in the list of substances of very high concern SVHC in the EU REACH annex, and it required that ADC resid...

AI Technical Summary

Problems solved by technology

Due to the current process and technology, it is still difficult to control and ensure that the content in leather products does not exceed the limit. Since azodicarbonamide was included in the SVHC list of substances of very high concern in the EU REACH Annex, it has been exported to foreign countries in the past two years The leather and its products are required to test the azodicarbonamide project

[0004] However, the key problem of the current regulatory difficulty is that there is no detection method, and there are very few domestic studies on the determination of ADC residues in leather, mainly due to the properties of azodicarbonamide itself. First, it is due to its chemical properties that decompose rapidly at high temperatures , so that the conventional quantitative instruments gas chromatography (GC) and gas chromatography (GC-MS, GC-MS-MS) can not be used, the second is its poor solubility makes the conventional quantitative instrument liquid mass spectrometry (LC -MS, LC-MS-MS) are excluded again, also make the mobile phase selection room of liquid chromatography greatly reduce at the same time, the 3rd, the strong polarity of this compound makes almost no retention on the reversed-phase column, brings to detection very difficult

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