Annatto oligosaccharide, preparation method and usage thereof

A technology of annatto and oligosaccharides, applied in the field of annatto oligosaccharides and its preparation and application, to achieve high product purity, simple preparation methods, and the effect of improving gastrointestinal function

Inactive Publication Date: 2014-03-26
NCPC NEW DRUG RES & DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Annatto oligosaccharides are new functional oligosaccharides, and there are no relevant research reports at home and abroad

Method used

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  • Annatto oligosaccharide, preparation method and usage thereof
  • Annatto oligosaccharide, preparation method and usage thereof
  • Annatto oligosaccharide, preparation method and usage thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment 1 Preparation method one of annatto oligosaccharide

[0031] Add 1kg of annatto seeds to 3L of 6% potassium hydroxide solution, stir at 50°C for 2 hours to degrease, pulverize the degreased annatto seeds, then use 6L of water for ultrasonic extraction for 2 hours, and concentrate the extract to obtain annatto oligosaccharides ( A) (wherein the annattoose content is 43.4%) (see Figure 7 ).

Embodiment 2

[0032] Embodiment 2 The preparation method two of annatto oligosaccharides

[0033] 1 kg of depigmented annatto seeds were pulverized, decocted and extracted with 3 L of water for 1 hour, and the extract was concentrated to obtain annatto oligosaccharides (wherein the annattoose content was 45.2%).

Embodiment 3

[0034] Embodiment 3 Preparation method three of annatto oligosaccharides

[0035] Add 1kg of annatto seeds to 1L of dichloromethane, degrease them by ultrasonication for 2 hours, decoct the degreased annatto seeds with 1L of water, decoct 3 times, 2 hours each time, concentrate the decoction, add 50ml of water to dilute, and conduct activated carbon Column chromatography (the consumption of active carbon is 0.5kg), successively eluted with water, 8%, 10%, 15%, 18%, 25% aqueous ethanol, detected by HPLC, combined 10%-18% ethanol eluate, Concentrate to obtain annatto oligosaccharides (wherein the annattoose content is 58.4%).

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Abstract

The invention relates to an annatto oligosaccharide which contains annatto tetrose with the mass of more than 40% of the total mass, wherein the annatto tetrose has the structural formula of alpha-D-Gal-(1-3)-alpha-D-Gal-(1-6)-alpha-D-Glc-(1-2)-beta-D-Fru. The preparation method of the annatto oligosaccharide and the annatto tetrose is simple in structure and high in product purity, has the activity of strengthening the immunity, adjusting intestinal flora and improving gastrointestinal tract functions, and is free from toxic side effect.

Description

technical field [0001] The invention relates to an annatto oligosaccharide extracted from natural plants, a preparation method and application thereof. technical background [0002] Bixo orellana, also known as mahogany, is a fast-growing small tree of the Mahogany family Mahogany, native to tropical America and the West Indies, mainly in Mexico, Brazil, Guyana and India. The yellow-orange pigment extracted from the fleshy outer testa of its small pyramid-shaped seeds is called annatto, and it has always been the dominant variety used for cream and cheese coloring in Western countries. With the revelation and understanding of the biological functions of sugar chains, glycobiology is becoming a new frontier of science. After genetic engineering and protein engineering, glycoengineering has become the most eye-catching new biotechnology discipline. The research on oligosaccharides is Major component in sugar engineering. Functional oligosaccharides, as new physiologically ac...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07H3/06A23L33/00
Inventor 任风芝陈书红张雪霞王秒郭栋耿文飞李丽红李宁白玉高月麒王宁张华
Owner NCPC NEW DRUG RES & DEV
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