Fluorescent quantum dot marked chitosan DNA nano composite vector and preparation and application thereof

Abstract

The invention discloses a fluorescent quantum dot marked chitosan DNA nano composite vector and preparation and application thereof, and particularly aims at transduction of jatropha curcas genes. The method comprises the following steps of: preparing CdSe fluorescent quantum dots with good water solubility and good biological affinity, and preparing the chitosan DNA nano vector by using a complex coacervation method. The quantum dots are combined to the chitosan DNA nano vector under the action of static electricity to form a fluorescent nano complex using the vector as a center and encircled by the quantum dots. The preparation method is simple and feasible; the obtained complex is uniform and has enhanced fluorescent property and good stability; and when the complex is used for the transduction of jatropha curcas cells, fluorescent expression signals of intracellular report genes (GFP protein) can be successfully detected, and the complex can be widely used for the transduction of other plant genes.

Description

technical field [0001] The invention belongs to the field of applied nano-biology technology, and relates to a fluorescent quantum dot-labeled chitosan DNA nanocomposite carrier and a preparation method thereof, as well as a method applied to jatropha curcas gene transduction. Background technique [0002] Nanocarriers have certain advantages in mediating gene transfer: good biological safety, biodegradability, low toxicity and immunogenicity; protection of genes from damage by various complements and enzymes; special structure and surface charge; Surface energy coupling of specific targeting molecules. Many experts and scholars at home and abroad have tried to use nanomaterials as carriers for exogenous gene transformation and gene therapy in animal cells, and have made gratifying progress. Recently, some scientists have also conducted exploratory research on the use of nanocarriers for plant gene transformation. Botanists and chemists at Iowa State University have succes...

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Problems solved by technology

At present, there are few reports on gene transduction research on fluorescent compound-labeled vectors

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