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Method for reserving, conserving and culturing daphnia magna

A cultivation method and technology of Daphnia daphnia, applied in fish farming, application, climate change adaptation, etc., can solve the problems of heavy workload, unfavorable preservation of pure species, cumbersome operation, etc., and achieve low cost, good water quality, and source of materials wide effect

Inactive Publication Date: 2011-08-03
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

If the experimental culture method is adopted for the laboratory conservation of Daphnia magna, the operation is cumbersome, the workload is large, and it is not conducive to the preservation of pure species.

Method used

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  • Method for reserving, conserving and culturing daphnia magna
  • Method for reserving, conserving and culturing daphnia magna

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0037] 1. Test material

[0038] 1 moss mouse, 2 snails, 5L round glass tank, about 50 large daphnia, 1×10 6 Left and right Scenedesmus, 4L tap water aerated for 3 days, 70cm siphon, 1 glass rod, 1 straw.

[0039] 2. Test process

[0040] Time: 2010.01-2010.12.

[0041] Temperature: 10-35°C

[0042] (1) Pour the aerated tap water into a 5L round glass tank, and inoculate 1×10 6 About 1 / L Scenedesmus, 50 Daphnia magna young, 2 snails and 1 moss rat. Placed on a test bench, out of direct sunlight, at room temperature. Observe that there is no abnormality in the system.

[0043] (2) Feed 20ml of 1×10 per week 6 Scenedesmus per L once to supplement the consumption of Scenedesmus magna. Keep various organisms in the ecosystem in the following relationship: the density of Scenedesmus is 4×10 3 ~6×10 3 A / L or so.

[0044] (3) Change the water every 7-10 days, and the water change volume is 8-10%.

[0045] (4) When there are a lot of residual impurities in the water body o...

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Abstract

The invention belongs to the field of culturing of zooplanktons and relates to an ecological method for reserving, conserving and culturing daphnia magna. In the method for reserving, conserving and culturing daphnia magna, a simple daphnia magna-alga-spiral-fish aquatic ecosystem needs to be established, the daphnia magna stably grows and is reproduced in the ecosystem, a small amount of culturing water is replaced periodically and a small amount of bait is added, so that the daphnia magna is stabilized in the system permanently. Along with wide application of toxicological testing of daphnia magna, daphnia magna needs to be provided at any time to serve as a testing material. A method for establishing a simple aquatic ecosystem is adopted, so that the operation is simple, the cost is low, the daphnia magna can stably grow and be reproduced, frequent field seed collecting is not needed, the troubles of frequency manual water change, bait feeding and clearing of impurities and waste during ordinary culturing are eliminated, and the aim of easily and conveniently preserving in a laboratory for a long time is fulfilled.

Description

technical field [0001] The invention belongs to the field of zooplankton cultivation, and relates to a method for cultivating ecological Daphnia daphnia reserve species. Background technique [0002] Daphnia large ( Daphnia magna ) lives in natural waters, belongs to planktonic crustaceans, and is the world species. It has the advantages of short life cycle, fast reproduction, economy, and sensitivity to poisons. It plays an important role in aquatic ecosystems and can be used to comprehensively evaluate the toxicity of industrial wastewater, pesticide toxicity, and new chemical substances. Daphnia magna is an internationally recognized standard test organism and is widely used in aquatic toxicity tests. [0003] With the development of daphnia magna toxicity test research, countries have successively established standard methods for the determination of daphnia magna toxicity, and made relevant regulations on the cultivation conditions of experimental daphnia magna, but t...

Claims

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Application Information

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IPC IPC(8): A01K61/00
CPCY02A40/81
Inventor 黄建荣窦碧霞
Owner SUN YAT SEN UNIV
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