Dialysis membrane fixing device

Abstract

The invention provides a dialysis membrane fixing device comprising a permanent seat and a jacket, wherein the permanent seat is a hollow cylinder; the jacket is clamped on the outer side wall of the permanent seat; the outer side wall of the permanent seat and the jacket are provided with holes of which the positions correspond; when the fixing device is used, a dialysis membrane cut into a stripy shape is unfolded to be clamped between the outer side wall of the permanent seat and the jacket; the upper end of the permanent seat is connected with a movable sleeve by virtue of a fastening ring and a seal ring; the inner bottom and outer bottom of the permanent seat are respectively provided with a magnetic stir bar; the lower end of the permanent seat is provided with supporting legs; andthe upper end of the movable sleeve is provided with a sample feeding hole and a sample feeding hole cover. The dialysis membrane fixing device can be used for greatly improving the dialysis efficiency, and is favorable for samples to remain in an aseptic condition in the dialysis and transfer process. By utilizing the dialysis membrane fixing device, the dialysis under the positive pressure and negative pressure condition can be carried out, and the dialysis membrane cut into the stripy shape is easy to clean, so that the use times are increased, and the cost is lowered.

Description

technical field [0001] The invention relates to a dialysis apparatus, especially a device for clamping and fixing a dialysis membrane for dialysis. Background technique [0002] Dialysis is one of the commonly used techniques in the laboratory, which is used for the replacement of buffer systems, crude purification, concentration, desalination, etc. of macromolecular substances such as proteins. The core component of dialysis technology is the dialysis membrane. The dialysis membrane with micropores allows molecules smaller than the diameter of the micropores to diffuse to the other side of the dialysis membrane, so that dialysis can proceed. At present, the laboratory generally uses dialysis membranes made of cellulose, mixed cellulose and other materials. The dialysis membranes are made into dialysis bags and dialysis tubes. During dialysis, the sample liquid is put into them, sealed and placed in the dialysate for dialysis. Dialysis bags and dialysis tubes are very conve...

AI Technical Summary

Problems solved by technology

However, the movement mode of the sample molecules in the dialysis bag and dialysis tube is free diffusion. After dialysis for a period of time, the concentration difference of the target molecules close to the dialysis membrane is close to zero, and the dialysis almost stops, while the concentration of the target molecules far away from the dialysis membrane is almost zero. change; when the target molecule increases the concentration difference near the dialysis membrane through diffusion, dialysis resumes again, so the dialysis efficiency is not fully exerted

When the diameter of the dialysis bag and dialysis tubing is large, the effect of uneven concentration difference becomes very significant, the dialysis efficiency is significantly reduced, and the time required to reach complete equilibrium is very long; in addition, the diffusion rate of large molecular weight target molecules is small, even if it is Dialysis of small samples also takes a long time to reach full equilibrium

The uneven concentration difference in the dialysis process is the biggest defect of the dialysis bag and dialysis tube. In clinical application, this problem is solved by keeping the sample flowing in a closed environment composed of dialysis membrane, pipeline and pump. However, the current laboratory There is no corresponding product

[0003] Some other defects of dialysis bags and dialysis tubes include: (1) Dialysis bags usually sink to the bottom of the dialysate after being sealed with a sealing clip. When there are many dialysis bags, the stirrer in the dialysate usually cannot rotate normally. Although the dialysis tube does not have this problem , but the cost is high; (2) The dialysis bags and dialysis tubes are not easy to clean after use. They can only be cleaned by repeated washing with running water. The cleaning effect is poor, and samples often remain on the inside of the membrane. Bags and dialysis tubes will increase the cost of experiments and are not environmentally friendly; (3) The mechanical strength of dialysis bags and dialysis tubes is not high, and they cannot be used under some environmental conditions that require pressurization or negative pressure; (4) Dialysis bags, dialysis tubes After the tube is aseptically treated, the sterility is not easy to maintain during use, especially during the sample transfer process, it is prone to contamination

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