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Method for batch preparation of chicken Eimeria coccidium oocysts

A technology of Eimeria chicken coccidia and oocysts, which is applied in the direction of protozoa, etc., can solve the problems of complicated sucrose density gradient centrifugation process, inconformity with chicken coccidia vaccine production conditions, and difficult preparation conditions, so as to save separation time , process simplification and cost reduction

Active Publication Date: 2012-08-22
内蒙古元本生物医药科技有限公司
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AI Technical Summary

Problems solved by technology

[0060] The saturated chromium sulfuric acid flotation method not only requires a large amount of centrifugation, but also requires ice bath conditions, which obviously do not meet the production conditions of chicken coccidiosis vaccines, and the cost is too high
[0061] The process of sucrose density gradient centrifugation is complicated, and the conditions are difficult to prepare. The separation process includes the saturated brine flotation method, and the shortcomings will not be repeated.

Method used

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Embodiment Construction

[0078] Take 20kg of fresh feces from infected chickens, add 60 liters of water to fully mix, add 1000mg of pepsin, mix well, and let it stand for 10 minutes; then add 140 liters of water and mix well to form feces; the total amount of feces is about 220 liters; start the continuous screening machine , Add the manure liquid from the feeding port at a constant speed (10-15 liters / minute), add 20 liters of water after the manure liquid is added, and keep the sieving machine running at the same time, so as to wash as much as possible the entrapped in the first, second, and third layers of screens unfiltered oocysts, then remove the 4th layer of sieve, wash the retentate pile with water, and collect it in a graduated container for later use; add 5 times (V:V) saturated brine according to the retentate amount, mix and float, and pack In a 500ml centrifuge tube, centrifuge at 2500rpm×10min; suck out the floating matter on the surface of the centrifuge tube and add at least 10 times of...

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Abstract

The invention discloses a method for batch preparation of chicken Eimeria coccidium oocysts, and particularly relates to a separation and purification method for producing oocysts (antigens) of chicken Eimeria coccidium vaccines. The method for preparing the chicken Eimeria coccidium oocysts comprises the following process steps: fecal sample processing, continuous screening, saturated brine float centrifuging, oocyst centrifugal depositing and oocyst purifying. According to the method, the industrial preparation of the chicken Eimeria coccidium oocysts can be realized, the production efficiency can be improved, and the cost can be reduced.

Description

technical field [0001] The invention discloses a method for preparing Eimeria oocysts in batches, and relates to a method for separating and purifying oocysts (antigens) for producing Eimeria chicken vaccines. Background technique [0002] About chicken Eimeria coccidiosis [0003] Eimeria (Eimeria) coccidium (Coccidium) is a class of obligate parasitic protozoa that parasitizes in chicken intestines, causing chicken coccidiosis (Coccidiosis). According to statistics, there are 15 kinds of chicken coccidia reported in the whole world, wherein, 5 kinds have obvious pathogenicity, they are: Eimeria tenella (E.tenella), poisonous Eimeria (E. tenella) .necatrix), E.maxima, E.acervulina, and E.mitis. Among the 5 pathogenic coccidia, 3 are harmful to chicks: Eimeria tenella, Eimeria giga, and Eimeria spp. [0004] Coccidiosis is a ubiquitous infectious disease. Where there are chickens in the world, there will be chicken coccidiosis, and there will be coccidiosis. Large-scale c...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/10
Inventor 郝文华郑润宽徐勇
Owner 内蒙古元本生物医药科技有限公司
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