Production method of microbial inoculum

A microbial agent and production method technology, applied in the field of microbial agent production, can solve the problems of not being able to meet the needs of the market, short survival time of live bacteria, limited storage time, etc., to promote rapid reproduction, growth and reproduction, The effect of increasing fertilizer efficiency

Active Publication Date: 2011-09-07
SHANGHAI NUOTONG INFORMATION TECH CO LTD
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  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

An authoritative department estimates that in the next five years, the annual demand for biological fertilizers will reach 30% of the amount of chemical fertilizers, and the market capacity will reach 42 million tons. However, the current annual production of biological fertilizers in my country is less than 3 million tons, which is far from being able to meet market needs
[0006] 3. No nutrition, short survival time of live bacteria: peat and differentiated coal do not contain the nutrients needed by live bacteria, so the compound microbial liquid is directly inoculated on peat, differentiated coal, rice husk and other carriers. Over time, the number of viable bacteria will gradually decrease, and the storage time is limited

Method used

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  • Production method of microbial inoculum
  • Production method of microbial inoculum

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Experimental program
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Effect test

Embodiment 1

[0045] 1. Strain fermentation

[0046] The three strains of Bacillus subtilis, Bacillus megaterium and Bacillus mucilginosus krassilm were respectively subjected to slant culture, shaker expansion culture, seed tank fermentation and fermenter fermentation , the number of live bacteria in the fermentation liquid of Bacillus subtilis reached 5.3 billion / ml, the number of live bacteria in the fermentation liquid of Bacillus megaterium reached 4.6 billion / ml, and the number of live bacteria in the fermentation liquid of Bacillus peptiformis Reached 2.8 billion / ml;

[0047] 2. Sampling and processing

[0048] The hydrolyzed slag was obtained from Henan Lianhua Monosodium Glutamate Co., Ltd.

[0049] Crush the large pieces of hydrolyzed slag into powder or small particles, mix with powdered lime according to the ratio of hydrolyzed slag:lime=4:1, pulverize, pile up the finely powdered material for 12-20 hours, and then pulverize for the second time. Then sieve and pack to make th...

Embodiment 2

[0059] The three strains of Bacillus subtilis, Bacillus megaterium and Bacillus mucilginosus krassilm were respectively subjected to slant culture, shaker expansion culture, seed tank fermentation and fermenter fermentation , the number of live bacteria in the fermentation liquid of Bacillus subtilis reached 5.3 billion / ml, the number of live bacteria in the fermentation liquid of Bacillus megaterium reached 4.6 billion / ml, and the number of live bacteria in the fermentation liquid of Bacillus peptiformis Reached 2.8 billion / ml;

[0060] Crush the large pieces of hydrolyzed slag into powder or small particles, mix with powdered lime according to the ratio of hydrolyzed slag:lime=5:1, pulverize, pile up the powdered material for 12-20 hours, and then sieve and pack into bags. Its pH reaches 5.5-7.0.

[0061] Bacillus subtilis (Bacillus subtilis), Bacillus megaterium (Bacillus megaterium) and Bacillus mucilginosus krassilm (Bacillus mucilginosus krassilm) were respectively adso...

Embodiment 3

[0065] Bacillus subtilis (Bacillus subtilis) and Bacillus megaterium (Bacillus megaterium) were separately cultured on a slant, expanded culture on a shaker, fermented in a seed tank and fermented in a fermenter. The number of live bacteria in the fermentation liquid of Bacillus subtilis Reached 5.3 billion / ml, and the number of live bacteria in the fermentation liquid of Bacillus megaterium reached 4.6 billion / ml;

[0066] Crush the large pieces of hydrolyzed slag into powder or small particles, mix with powdered lime according to the ratio of hydrolyzed slag:lime=5:1, pulverize, pile up the powdered material for 12-20 hours, and then sieve and pack into bags. Its pH reaches 7.0-7.5.

[0067] Bacillus subtilis (Bacillus subtilis) and Bacillus megaterium (Bacillus megaterium) are adsorbed with the hydrolyzed slag that has been treated respectively, and the bacterium liquid of single bacterial species is according to bacterium liquid (L): hydrolyzed slag (Kg)= The ratio of 0.3...

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Abstract

The invention provides a production method of microbial inoculum and the method contains the fermentation and adsorption of the strain. The method comprises the following steps: utilizing treated hydrolysis residue as a bacterium solution adsorption medium to absorb the fermented bacterium solution of one or more of Bacillus subtilis, Bacillus megaterium and Bacillus mucilaginous krassilm, wherein the weight ratio of the bacterium solution to the hydrolysis residue is 0.3:(2-4); and then mixing and crushing, thus obtaining the microbial inoculum. The invention provides a new powdery microbial inoculum adsorption carrier, and the carrier contains various nutrients for microbial growth, is favorable for microbial proliferation and growth and is environmentally-friendly and low in price.

Description

technical field [0001] The present invention relates to a production method of microbial inoculum, in particular, relates to a method of using glutamic acid or monosodium glutamate (glutamic acid is the main raw material for producing monosodium glutamate) produced in the production process of hydrolyzed slag as a microbial inoculum adsorption carrier Production method of microbial inoculant. Background technique [0002] Soil not only provides essential nutrients and water for plants, but also is the habitat for soil animals to survive. However, due to the application of a large amount of chemical fertilizers, the enrichment of inorganic elements in the soil increases, and the accumulation of organic matter and beneficial microorganisms decreases rapidly, resulting in soil compaction. , the quality has declined, the ability to retain water and fertilizer has decreased, agricultural products have been seriously polluted, and the entire farmland ecological environment and peop...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C05F11/08C12R1/125C12R1/11C12R1/07
Inventor 闫龙翔刘西莉陈露
Owner SHANGHAI NUOTONG INFORMATION TECH CO LTD
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