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Bovine colostrum product resisting infantile diarrhea

A technology for infantile diarrhea and dairy products, applied in dairy products, milk preparations, applications, etc., can solve the problems of unsatisfactory treatment of group A rotavirus, unsatisfactory active immunization effect, and unused human beings, so as to achieve drug resistance Strains, less side effects, and simple preparation methods

Inactive Publication Date: 2012-09-19
江西英雄乳业股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004]Currently, the treatment of group A rotavirus (ARV) is not ideal. At present, supportive therapy is mostly used, and the effect of active immunization is not ideal
In recent years, some scholars have fed mice with anti-ARV chicken yolk immunoglobulin (anti-ARV IgY for short), and the mice have obtained passive immune protection, but it has not been used in humans

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] The preparation of group A rotavirus (ARV) antigen has the following steps:

[0047] (1) Culture of MA104 cells

[0048] Take out a bottle of preserved MA104 cells from the liquid nitrogen tank, thaw quickly at 37°C, inoculate into a culture bottle in a sterile room, and place in 37°C CO 2 incubator cultivation;

[0049] Preparation of culture medium: Dissolve 9.5g of MEM powder (GIBCO) into 850mL of five-distilled water; add 2% L-glutamine, 0.15mol sodium pyruvate, and double antibodies (10,000 units of Penicillium and 12,500 units of streptomycin) The mother liquor was added to 850mL MEM solution at 1% respectively; the volume was adjusted to 1000mL, sterilized by suction, aliquoted, and stored at -20°C.

[0050] (2) Cultivation of rotavirus

[0051] Take 1 mL of human-Wa strain rotavirus seed and add trypsin to a final concentration of 10 μg / mL, and react at 37 °C for 30 min; wash the culture flask of MA-104 cells that have adhered to the wall with D-Hanks solutio...

Embodiment 2

[0063] The preparation of group A rotavirus (ARV) antigen has the following steps:

[0064] (1) Culture of MA104 cells

[0065] Take out a bottle of preserved MA104 cells from the liquid nitrogen tank, thaw quickly at 37°C, inoculate into a culture bottle in a sterile room, and place in 37°C CO 2 incubator cultivation;

[0066] Preparation of culture medium: Dissolve 9.0g of MEM powder (GIBCO) into 850mL of five-distilled water; add 2% L-glutamine, 0.15mol sodium pyruvate, and double antibodies (10,000 units of Penicillium and 12,500 units of streptomycin) The mother liquor was added to 850mL MEM solution at 1% respectively; the volume was adjusted to 1000mL, sterilized by suction, aliquoted, and stored at -20°C.

[0067] Among them, the immunization procedure of group A rotavirus (ARV) antigen is as follows: intramuscular injection in the neck; Freund's complete adjuvant is used for the first immunization, and Freund's incomplete adjuvant is used to replace the complete adj...

Embodiment 3

[0070] The preparation of group A rotavirus (ARV) antigen has the following steps:

[0071] (2) Culture of MA104 cells

[0072] Take out a bottle of preserved MA104 cells from the liquid nitrogen tank, thaw quickly at 37°C, inoculate into a culture bottle in a sterile room, and place in 37°C CO 2 incubator cultivation;

[0073] Preparation of culture medium: Dissolve 10.0g of MEM powder (GIBCO) into 850mL of five-distilled water; add 2% L-glutamine, 0.15mol sodium pyruvate, and double antibodies (10,000 units of Penicillium and 12,500 units of streptomycin) The mother liquor was added to 850mL MEM solution at 1% respectively; the volume was adjusted to 1000mL, sterilized by suction, aliquoted, and stored at -20°C.

[0074] Among them, the immunization procedure of group A rotavirus (ARV) antigen is as follows: subcutaneous injection around the breast; Freund's complete adjuvant is used for the first immunization, and Freund's incomplete adjuvant is used to replace the comple...

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Abstract

The invention relates to a bovine colostrum product resisting infantile diarrhea, in particular to a bovine colostrum product resisting rotavirus. The bovine colostrum product is achieved by the steps as follows: preparation of group A rotavirus (ARV), immunization of group A rotavirus (ARV) antigen, collection of immune milk, and preparation of milk product by the immune milk. The prepared bovine colostrum product resisting infantile diarrhea in the invention not only has a very good effect on preventing infantile diarrhea but also has the advantages of being excellent in specificity, small in side effects, simple and convenient in preparation method, easy for industrialization, not apt to generate drug-resistant strains, and the like.

Description

technical field [0001] The invention relates to a bovine colostrum product against infantile diarrhea, in particular to a bovine colostrum product against rotavirus. Background technique [0002] Group A rotavirus (ARV) mainly infects infants and young children, and is the main pathogen causing viral enteritis in infants and young children. According to the World Health Organization (WHO), more than 1 million children die from rotavirus diarrhea every year in the world. The first cause of infant diarrhea death in China, from the end of 1999 to the beginning of 2000, there was a large-scale outbreak of rotavirus diarrhea in infants and young children in Beijing, Shenzhen and other places in my country. [0003] Immune milk refers to selectively inoculating mammals (mainly cows, goats, etc.) with some bacteria, viruses or other foreign antigens (Antigen) that can cause human or animal diseases, and stimulates the body to generate an immune response to secrete specific antibodie...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23C9/20
Inventor 江小芬
Owner 江西英雄乳业股份有限公司
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