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Extracting and purifying method for lovastatin
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A technology of lovastatin and pure product is applied in the field of extraction and purification of lovastatin, which can solve the problems of high cost, large environmental pollution and large amount, and achieves the effect of solving low extraction efficiency.
Active Publication Date: 2014-05-07
CHONGQING RUEPEAK PHARMA
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The solvent extraction method consumes a large amount of solvent, the cost is high, and the yield is low after repeated extractions. The macroporous adsorption resin can be used repeatedly, but the use of the macroporous adsorption resin requires the use of a large amount of acid and water, which is harmful to the environment. Big pollution
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Embodiment 1
[0013] The selection of embodiment 1 entrainer
[0014] Take by weighing 5 parts of 100.0 g of dried fermented liquid bacterial residue, use methanol, ethanol, ethylene glycol and ethyl acetate as entrainer respectively to extract, and take one part without entrainer as contrast;
[0015] Fill the above-mentioned fermentation liquid bacteria residue into the supercritical extraction kettle, and pressurize and heat up according to the program, and carry out dynamic extraction, CO 2 The flow rate is 4.6 L h -1 , the extraction temperature is 55°C, the extraction pressure is 25MPa, and the entrainer dosage is 2mL·g -1 , quantitative pump into the entrainer, the extraction time is 100min; collect the product from the analysis kettle, reclaim the organic solvent under reduced pressure, and obtain the yellow solid crude extract, the crude extract is ultrasonically washed 3 times with 95% ethanol, each 10ml, The washing time was 15min, 10min, and 10min respectively, and the s...
Embodiment 2
[0020] The selection of embodiment 2 washing solvents
[0021] Weigh 5 parts of 100.0g dried fermentation broth bacteria residues, fill them into the supercritical extraction kettle respectively, pressurize the temperature according to the program, and carry out dynamic extraction, CO 2 The flow rate is 4.6 L h -1 , the extraction temperature is 55°C, the extraction pressure is 25MPa, absolute ethanol is used as the entrainer, and the entrainer dosage is 2mL·g -1 , quantitatively pump the entrainer, and the extraction time is 100min; the product is collected from the analysis kettle, the organic solvent is recovered under reduced pressure, and the yellow solid crude extract is obtained, and the concentration is absolute ethanol, 95% ethanol, 85% ethanol, 75% Ethanol and 55% ethanol ultrasonically washed the crude extract 3 times, 10ml each time, the extraction time was 15min, 10min, 10min respectively, and the solid part was obtained by suction filtration; the specific resu...
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Abstract
The invention provides an extracting and purifying method for lovastatin, which employs SFE (supercritical fluid extraction)-CO2 to extract fermentation fungus residues and uses ethanol to wash a crude product. By combining SFE-CO2 with organic phase solid washing of the crude product, and using absolute ethane as an entrainer and 95% ethanol as a washing agent, the method solves the problems that the traditional extraction modes of lovastatin have low extraction efficiency, high labor intensity and high environmental pollution. More importantly, the purity of lovastatin acquired by using the method is higher than 98%, and the method can completely substitute for the traditional chromatographic separation method and macroporous resin adsorption extraction method.
Description
technical field [0001] The invention relates to a method for extracting chemical components, in particular to a method for extracting and purifying lovastatin. technical background [0002] Lovastatin (1ovastatin) is a clinically important hypolipidemic drug, and it is a hydroxymethylglutaryl coenzyme A (HMG-CoA) reductase inhibitor. It can reduce the synthesis of cholesterol by inhibiting HMG-CoA reductase, and up-regulate the expression of low-density lipoprotein receptors on the surface of liver cells, and accelerate the transfer and metabolic clearance of low-density lipoprotein cholesterol in the blood to the liver, so as to achieve the effect of lowering blood lipids. effect. Lovastatin is mainly a secondary metabolite of fungi. [0003] Lovastatin is a weakly polar substance, hardly soluble in water, soluble in lower alcohols, ketones, ethyl acetate, butyl acetate, benzene, toluene, insoluble in petroleum ether, n-hexane, and generally used for solvent extraction. ...
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