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Application of mannitol in preparing antitumor medicines

An anti-tumor drug, mannitol technology, applied in the direction of anti-tumor drugs, drug combination, drug delivery, etc., can solve the problem that there is no clinical application precedent of mannitol anti-tumor treatment

Inactive Publication Date: 2011-10-26
广西壮族自治区肿瘤防治研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no report on whether mannitol can induce apoptosis of cancer cells at home and abroad, and there is no precedent of clinical application of mannitol for anti-tumor therapy

Method used

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  • Application of mannitol in preparing antitumor medicines
  • Application of mannitol in preparing antitumor medicines
  • Application of mannitol in preparing antitumor medicines

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] The basic experimental research of embodiment 1 mannitol anti-tumor (lung cancer)

[0036] 1 Experimental materials

[0037] 1.1 Materials

[0038] 1.1.1 Cell line: SPC-A-1 human lung cancer cell line was obtained from Shanghai Cancer Institute.

[0039] 1.1.2 Reagents: RPMI 1640 and trypsin were purchased from Gibcol BRL; newborn bovine serum was purchased from Hangzhou Sijiqing Bioengineering Materials Co., Ltd.; sodium bicarbonate (analytical grade) was produced by Hebei Cizhou Pharmaceutical Factory; sodium penicillin and streptomycin sulfate Su is produced by Harbin Pharmaceutical Group Pharmaceutical General Factory; thiazolyl blue (MTT) and dimethyl sulfoxide (DMSO) are products of Sigma; ATP kits (ATP extract, luciferin-luciferase, diluent, etc.) are German DCS The company's products; sodium citrate was produced by Guangzhou Chemical Reagent Factory; propidium iodide (PI) and RNaseA were products of Sigma; mannitol injection was purchased from Guangxi Nanning ...

Embodiment 2

[0056] Example 2 Study on the Apoptosis and Mechanism of Lung Adenocarcinoma Cells Induced by Specific Concentrations of Mannitol (Lung Adenocarcinoma)

[0057] 1 Materials and methods

[0058] 1.1 Main reagents and instruments: DMEM (high sugar) culture medium (subpackaged by Thermo Fisher Biochemical Beijing Co., Ltd., a hyclone company in the United States), trypsin, newborn calf serum (FBS was purchased from Hangzhou Sijiqing Biological Co., Ltd.) , 20% mannitol (purchased from Anshuanghe Pharmaceutical Co., Ltd.), Giemsa staining kit (provided by the Testing Center of the First Affiliated Hospital of Guangxi Medical University), cell apoptosis hoechst 33342 kit (purchased from Shanghai Biyuntian Biotechnology Co., Ltd. Co., Ltd.) apoptosis Dapi staining kit, flow cytometry kit (both purchased from Nanjing Kaiji Biotechnology Development Co., Ltd.), Bcl-2 detection kit (purchased from Fuzhou Maixin Biotechnology Development Co., Ltd.), Pentylene glycol and transmission el...

Embodiment 3

[0098] Embodiment 3 breast cancer

[0099] Referring to Example 1, the MTT method was used to determine the cell inhibitory rate of a specific concentration of mannitol on breast cancer cells. The results showed that within 48 hours, mannitol could inhibit the proliferation activity of breast cancer cells in the concentration range of 1-150 μg / μl, and its inhibitory effect was enhanced with the increase of concentration. Within 5 days, with the increase of concentration and time, the inhibition rate decreased Also increasing.

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Abstract

The invention discloses an application of mannitol in preparing antitumor medicines, and particularly relating to an application of a mannitol injection of a specific concentration (10-19%) in treating lung cancer, liver cancer, breast cancer, pancreatic cancer, stomach cancer, adenocarcinoma of stomach, and so on. Traditionally, mannitol is only used as a diuretic, a high-penetration antihypertensive agent, and so on. The inventor finds that mannitol has antitumor activity through scientific experiments, confirms that by using a multiterm apoptosis detection technique and detecting apoptosis-associated genes bc1-2 and bax, mannitol of a specific concentration can induce distinct apoptosis of a great variety of cancer cells, and masters the effect relationship between concentration and time, and preliminarily elucidates the possible mechanism. In addition, the inventor applies the research results in treating tumor patients and obtains distinct curative effects. The results in the invention provide theoretical basis and practical cases for the application of mannitol in preparing antitumor medicines and the clinical applications of mannitol.

Description

technical field [0001] The invention relates to a new application of mannitol, especially the application of mannitol in the preparation of antitumor drugs. Background technique [0002] At present, in anti-tumor research, inducing the apoptosis of cancer cells is an important way. Promoting tumor cell apoptosis can achieve the purpose of tumor shrinkage and cancer regression. For example, traditional anti-tumor drugs cisplatin and melphalan can all Stimulates the mitochondrial apoptotic pathway in cells, triggering apoptosis. This method has obvious advantages over the treatment method of killing tumor cells, because if anti-tumor drugs can induce the apoptosis of tumor cells in large quantities and activate their own programmed death process, it will avoid the release of tumor cells due to a large number of necrotic cells. Various side effects caused by intracellular substances can reduce the damage of chemotherapy to normal cells and prolong the survival of patients. Ho...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/047A61K9/08A61P35/00
Inventor 黄鼎铭刘德森黄亚铭岳惠芬潘琪李黄羿黎斌黄亚芬欧超崔英梁新强茅乃权左传田谢彤潘泓杨立黄耀元
Owner 广西壮族自治区肿瘤防治研究所
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