Polyaniline (PAn) modified ordered mesoporous carbon (EOMC) immobilized laccase biosensor and preparation method

A technology of biosensor and immobilized laccase, which is applied in the direction of instruments, scientific instruments, measuring devices, etc., can solve the problems of difficult electron transfer, inactivation, and deformation between enzymes and electrodes, and achieve good sensing performance, good repeatability and The effect of stability

Inactive Publication Date: 2011-11-23
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, enzymes usually have a large molecular weight, and the electroactive center of the enzyme molecule is surrounded by polypeptide chains, which hinders the electron transfer between the electrode and t

Method used

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  • Polyaniline (PAn) modified ordered mesoporous carbon (EOMC) immobilized laccase biosensor and preparation method
  • Polyaniline (PAn) modified ordered mesoporous carbon (EOMC) immobilized laccase biosensor and preparation method
  • Polyaniline (PAn) modified ordered mesoporous carbon (EOMC) immobilized laccase biosensor and preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Dissolve 2.5g epoxy resin in 10ml absolute ethanol, add 0.5g curing agent 2-ethyl-4-methylimidazole, stir for 2h, then add 2g SBA-15, stir well for 12h to make the polymer solution full Disperse in mesoporous silicon SBA-15, then place it in a vacuum drying oven at 100°C for 4 hours to remove the solvent; the mixture prepared above is protected by a N2 atmosphere with a flow rate of 100mL / min, and the temperature rise rate is 5°C / min. Carbonize at 950°C for 6h; finally, soak the resulting product with 40wt% hydrofluoric acid overnight to remove the template, then filter, wash with ethanol and deionized water, and dry at 100°C to constant weight to obtain EOMC. Do transmission electron microscope (TEM) analysis of the prepared EOMC, such as figure 1 As shown, perpendicular to the direction of the EOMC hole axis, it can be clearly seen that the channels are arranged in an orderly linear manner. It can be seen that the structure of EOMC has good long-range order, and it also...

Embodiment 2

[0054] Dissolve 2.5g epoxy resin in 8ml absolute ethanol, add 0.4g curing agent 2-ethyl-4-methylimidazole, stir for 2h, then add 2g SBA-15, stir well for 10h to make the polymer solution full Disperse in mesoporous silicon SBA-15, and then place it in a vacuum drying oven at 80°C for 3 hours to remove the solvent; the mixture prepared above is protected by a N2 atmosphere with a flow rate of 80mL / min, and the temperature rises at a rate of 4°C / min. Carbonize at 800°C for 5h; finally, soak the resulting product with 40wt% hydrofluoric acid overnight to remove the template, then filter, wash with ethanol and deionized water, and dry at 80°C to constant weight to obtain EOMC. Do transmission electron microscope (TEM) analysis of the prepared EOMC, such as figure 1 As shown, perpendicular to the direction of the EOMC hole axis, it can be clearly seen that the channels are arranged in an orderly linear manner. It can be seen that the structure of EOMC has good long-range order, and i...

Embodiment 3

[0067] Dissolve 2.5g epoxy resin in 12ml absolute ethanol, add 0.6g curing agent 2-ethyl-4-methylimidazole, stir for 3h, then add 2g SBA-15, stir well for 15h to make the polymer solution full Disperse in mesoporous silicon SBA-15, then place it in a vacuum drying oven at 100°C for 5 hours to remove the solvent; the mixture prepared above is protected by a N2 atmosphere with a flow rate of 100mL / min, and the temperature rises at a rate of 6°C / min. Carbonize at 1000°C for 8h; finally, soak the obtained product with 40wt% hydrofluoric acid overnight to remove the template, then filter, wash with ethanol and deionized water, and dry at 100°C to constant weight to obtain EOMC. Do transmission electron microscope (TEM) analysis of the prepared EOMC, such as figure 1 As shown, perpendicular to the direction of the EOMC hole axis, it can be clearly seen that the channels are arranged in an orderly linear manner. It can be seen that the structure of EOMC has good long-range order, and i...

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Abstract

The invention relates to a polyaniline (PAn) modified ordered mesoporous carbon (EOMC) immobilized laccase biosensor and a preparation method. The biosensor is characterized in that enzyme, EOMC+Lac and an EOMC+Lac/PAn modified gold electrode can be better immobilized by covering the periphery of mesoporous carbon with a layer of PAn film. The biosensor and the preparation method have the following beneficial effects: the gold electrode modified by the PAn modified EOMC immobilized laccase can show good direct electrochemical behavior; under the experimental conditions that the room temperature is 25+/-2 DEG C, the pH value of phosphate buffered solution is 5.0 and the working voltage is +0.45V vs SCE, compared with the EOMC+Lac/Au electrode which is not modified by PAn, the EOMC/PAn+Lac/Au electrode shows better sensing property; the linear detection range of p-pyrocatechol of the EOMC/PAn+Lac/Au electrode is 0.55-10.45mu M, the selective sensitivity of the EOMC/PAn+Lac/Au electrode is 0.08314A/M (n=19), the apparent Michaelis constant of the EOMC/PAn+Lac/Au electrode is 11.5327mu M and the detection limit of the EOMC/PAn+Lac/Au electrode is 0.173mu M (S/N=3); and besides, the laccase biosensor also shows good repeatability and stability; therefore, the PAn modified mesoporous carbon is expected to become a composite for immobilizing laccase and a new thinking is provided for modifying the immobilized enzyme by the mesoporous carbon.

Description

Technical field [0001] The invention relates to a biosensor of polyaniline modified ordered mesoporous carbon laccase and a preparation method. Background technique [0002] Laccase sensors can easily and quickly detect toxic substances such as phenols, aromatic amines, organophosphorus compounds and dioxins in wastewater, and have broad application prospects. Therefore, the study of laccase sensors with high sensing performance (such as high sensitivity, fast response, wide detection line and low detection line, etc.) has very important significance in environmental monitoring and detection. [0003] Enzyme electrode is the earliest and most mature type of biosensor, and its development has gone through three stages. The second-generation mediator-type enzyme electrode uses an artificially added electronic mediator to increase the chemical modification layer, which expands the range of the matrix electrode to detect chemical substances and also improves the sensitivity of the mea...

Claims

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Application Information

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IPC IPC(8): G01N27/327
Inventor 许鑫华路会冉郭美卿吴湘锋侯红帅
Owner TIANJIN UNIV
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