A new method to increase antibody levels of Vibrio anguillarum in turbot eggs

A technology of turbot and vibrio eel, applied in the field of immune antibodies, to achieve strong practicability, improve immune resistance, and reduce breeding losses

Inactive Publication Date: 2014-10-08
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the research on maternal immunity of turbot has not been reported so far. In view of this industrial background and research background, we researched and developed a new method to improve the antibody level of Vibrio anguillarum in turbot eggs

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Adopt above-mentioned technical scheme, take the Vibrio anguillarum bacterium liquid concentration range of immunization vaccine in the vaccine preparation and be 10 8 CFU / ml.

[0028] The prepared vaccine is injected intramuscularly to immunize the broodstock, the injection site is on the back of the fish body, and 3-5 points are selected for injection, and the injection dose is 0.2ml / kg of the broodstock body weight.

[0029] The described immunization condition is to immunize 60 days before broodstock spawn, and to immunize twice after the interval of 14 days after the first immunization.

[0030] The temperature range of the broodstock culture adopted is 11°C, and the broodstock is fed twice a day, the daily feeding amount is 1% of the fish body weight, the light is 200 lux, the light time is gradually increased from 8 hours to 18 hours, and the running water is cultivated. The daily exchange volume is 2 full doses.

[0031] Eggs produced by immunized female fish ...

Embodiment 2

[0033] Adopt above-mentioned technical scheme, take the Vibrio anguillarum bacterium liquid concentration range of immunization vaccine in the vaccine preparation and be 10 9 CFU / ml.

[0034] The prepared vaccine is injected intramuscularly to immunize the broodstock, the injection site is on the back of the fish body, and 3-5 points are selected for injection, and the injection dose is 0.3ml / kg of the broodstock body weight.

[0035] The described immunization condition is to carry out immunization 80 days before broodstock spawn, after the interval of 14 days after the first immunization, carry out two immunizations again respectively.

[0036] The temperature range of broodstock cultivation adopted is 13°C, fed twice a day, the daily feeding amount is 2% of the fish body weight, the light is 250 lux, the light time is gradually increased from 8 hours to 18 hours, the running water is cultivated, and the daily exchange amount is 4 full quantities.

[0037]Eggs produced by ...

Embodiment 3

[0039] Adopt above-mentioned technical scheme, take the Vibrio anguillarum bacterium liquid concentration range of immunization vaccine in the vaccine preparation and be 10 10 CFU / ml.

[0040] The prepared vaccine is injected intramuscularly to immunize the broodstock, the injection site is on the back of the fish body, and 3-5 points are selected for injection, and the injection dose is 0.4ml / kg of the broodstock body weight.

[0041] The immunization condition is to immunize 90 days before broodstock spawn, and to immunize twice after the interval of 15 days after the first immunization.

[0042] The temperature range of broodstock cultivation adopted is 15°C, fed twice a day, the daily feeding amount is 3% of the fish body weight, the light is 300 lux, the light time is gradually increased from 8 hours to 18 hours, the running water is cultivated, and the daily exchange volume is 6 full quantities.

[0043] Eggs produced by immunized female fish contained significantly hi...

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Abstract

The invention provides a new method for improving the Vibrio anguillarum antibody level in turbot eggs, which is characterized in that: (1) injecting the prepared vaccine to female turbot broodstock; (2) selecting female turbot broodstock Immunization is carried out in the period before spawning; (3) The cultivation conditions such as water temperature, light, and feeding are regulated, and management operations such as aeration and water change are adopted in time; Mature eggs containing high levels of Vibrio anguillarum antibodies were produced. The invention has beneficial effects: it can improve the immune resistance of the fertilized eggs of turbot, play a role in the disease prevention and control of early seedlings, reduce the breeding loss caused by diseases; avoid drug residues that may exist in the prevention and control of aquatic diseases by drugs , environmental pollution and other potential hazards; it is an effective way to realize the benefits of offspring through maternal immunization; it is easy to operate, strong in practicability, close to the actual production, and suitable for widespread promotion.

Description

technical field [0001] The invention relates to a method for increasing immune antibodies in eggs by immunizing broodstock mothers, in particular to a new method for increasing the level of Vibrio anguillarum antibodies in turbot eggs. Background technique: [0002] Turbot (Scophthalmus maximus) belongs to bony fish Pleuronectiformes, Pleuronectoidei, Bothidae and Scophthalmus. Its English name is turbot, commonly known as European flounder, and its transliteration name is " Turbot". Native to the east coast of the Atlantic Ocean, it is a rare cold-water economic fish in Europe. The optimum growth temperature is 14-17°C. The feeding habit of turbot in the natural environment is carnivorous, and the young fish eat crustaceans; the adult fish eat small fish, shrimp, etc. Male turbot reaches sexual maturity in one year, and female turbot reaches sexual maturity in two years. The natural maturity period is from May to August every year. Artificially cultivated broodstock can...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K61/00
CPCY02A40/81
Inventor 丁福红雷霁霖马爱军刘新富王蔚芳洪磊
Owner YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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