A new method to increase antibody levels of Vibrio anguillarum in turbot eggs
A technology of turbot and vibrio eel, applied in the field of immune antibodies, to achieve strong practicability, improve immune resistance, and reduce breeding losses
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Embodiment 1
[0027] Adopt above-mentioned technical scheme, take the Vibrio anguillarum bacterium liquid concentration range of immunization vaccine in the vaccine preparation and be 10 8 CFU / ml.
[0028] The prepared vaccine is injected intramuscularly to immunize the broodstock, the injection site is on the back of the fish body, and 3-5 points are selected for injection, and the injection dose is 0.2ml / kg of the broodstock body weight.
[0029] The described immunization condition is to immunize 60 days before broodstock spawn, and to immunize twice after the interval of 14 days after the first immunization.
[0030] The temperature range of the broodstock culture adopted is 11°C, and the broodstock is fed twice a day, the daily feeding amount is 1% of the fish body weight, the light is 200 lux, the light time is gradually increased from 8 hours to 18 hours, and the running water is cultivated. The daily exchange volume is 2 full doses.
[0031] Eggs produced by immunized female fish ...
Embodiment 2
[0033] Adopt above-mentioned technical scheme, take the Vibrio anguillarum bacterium liquid concentration range of immunization vaccine in the vaccine preparation and be 10 9 CFU / ml.
[0034] The prepared vaccine is injected intramuscularly to immunize the broodstock, the injection site is on the back of the fish body, and 3-5 points are selected for injection, and the injection dose is 0.3ml / kg of the broodstock body weight.
[0035] The described immunization condition is to carry out immunization 80 days before broodstock spawn, after the interval of 14 days after the first immunization, carry out two immunizations again respectively.
[0036] The temperature range of broodstock cultivation adopted is 13°C, fed twice a day, the daily feeding amount is 2% of the fish body weight, the light is 250 lux, the light time is gradually increased from 8 hours to 18 hours, the running water is cultivated, and the daily exchange amount is 4 full quantities.
[0037]Eggs produced by ...
Embodiment 3
[0039] Adopt above-mentioned technical scheme, take the Vibrio anguillarum bacterium liquid concentration range of immunization vaccine in the vaccine preparation and be 10 10 CFU / ml.
[0040] The prepared vaccine is injected intramuscularly to immunize the broodstock, the injection site is on the back of the fish body, and 3-5 points are selected for injection, and the injection dose is 0.4ml / kg of the broodstock body weight.
[0041] The immunization condition is to immunize 90 days before broodstock spawn, and to immunize twice after the interval of 15 days after the first immunization.
[0042] The temperature range of broodstock cultivation adopted is 15°C, fed twice a day, the daily feeding amount is 3% of the fish body weight, the light is 300 lux, the light time is gradually increased from 8 hours to 18 hours, the running water is cultivated, and the daily exchange volume is 6 full quantities.
[0043] Eggs produced by immunized female fish contained significantly hi...
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