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Device for separating peripheral blood mononuclear cells

A technique for nuclear cells and peripheral blood, which is applied in the field of devices for the separation of peripheral blood mononuclear cells, can solve the problems of easy cell death or destruction, poor stratification effect, etc., and achieve the effect of improving the cell separation effect.

Active Publication Date: 2013-02-06
FOURTH MILITARY MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, temperature control is also very important during the experiment. If the temperature is too high, the cells will easily die or be damaged. If the temperature is too low, the stratification effect will not be good.

Method used

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  • Device for separating peripheral blood mononuclear cells
  • Device for separating peripheral blood mononuclear cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] As shown in Figure 1, a kind of device that is used to separate peripheral blood mononuclear cell comprises a test tube rack 17, a control box 8, is provided with the cantilever frame 9 that hangs blood bag 10 above the control box 8, and is equipped with injection pump 5 and a transparent container 8 with a scale 6, a piston and a piston rod 1 are arranged in the transparent container 8, and one end of the piston rod 1 passing through the transparent container is connected to the syringe pump 5; the other end of the transparent container is provided with two connecting conduits, One is connected to the blood bag 10 for the pumping vessel 11 , and the other is connected to the shunt tube 14 for the transfusion vessel 13 . Both connecting conduits are provided with a one-way valve 8 to ensure that the blood flow in the pumping vessel 11 can only go in and out; the blood flow in the infusion tube 13 can only go out but not in. The control box 8 has a built-in power supply...

Embodiment 2

[0020] Increase the temperature adjustment button 5 on the front panel of the control box 7, and seal the other faces of the test tube rack 17 except the top to form a five-sided box body, which contains water, so that the inserted test tube 15 is bathed in water, and around the inner wall of the box The resistance wire is set to be connected to the temperature adjustment button 5, and the voltage applied by the resistance wire can be controlled by connecting the temperature adjustment button 5 to the controller, so as to ensure that the water temperature in the tank is at a constant temperature of 20°C-25°C. Therefore, the problem of easy cell death and destruction (too high temperature) or poor stratification effect (too low temperature) of the peripheral blood mononuclear cells can be solved.

Embodiment 3

[0022] On the basis of embodiment 2, increase flow rate detection (infrared drop number sensor, place on dropper 18, be responsible for blood flow rate detection) and temperature detection (temperature sensor, be placed in the water bath box integrated with test tube rack, be responsible for blood flow rate detection) water temperature detection), a display (placed on the front panel of the control box 7), a single-chip microcomputer is added in the control box 7, and the input of the single-chip microcomputer is the signal obtained by the flow rate detection and temperature detection, and the forward and reverse switch 3, the flow control button 4 1. The temperature adjustment button 5 is related to the setting signal of artificial input. The output of the single-chip microcomputer includes real-time flow rate, temperature signal (displayed on the display), flow rate control signal (to the controller to control the forward and reverse rotation and speed of the syringe pump), t...

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Abstract

The invention discloses a device for separating peripheral blood mononuclear cells, which comprises a test tube rack and a control box, wherein a cantilever for suspending a blood bag, an injection pump and a transparent container with scales are arranged above the control box; a piston and a piston rod are arranged in the transparent container; one end of the piston rod, which penetrates out of the transparent container, is connected with the injection pump; the other end is provided with two connecting ducts which are respectively connected with the blood bag and a diversion tube; the two connecting ducts are respectively provided with a one-way valve; a control box built-in power source and a controller are connected with the injection pump; the injection pump pulls the piston rod through a tumbler switch on the control box panel so as to extract blood into the transparent container, or pushes the piston rod to extrude the blood in the transparent container; the test tube rack is provided with at least one row of test tube plug holes, into which a plurality of test tubes are inserted in a 45-degree inclined mode; the diversion tube is arranged above the 45-degree inclined test tubes, and extends a dropping tube for each test tube hole; and each dropping tube is provided with a control valve.

Description

technical field [0001] The invention relates to a device for separating peripheral blood mononuclear cells (PBMC) experiments in medical teaching and scientific research experiments. Background technique [0002] In cell biology experiments, the determination of cellular immune function must first obtain active cells, such as lymphocytes, macrophages, granulocytes, etc., from peripheral blood or tissues of humans or animals. The methods used include density gradient centrifugation, adsorption separation and other special separation methods. No matter which method is used, the cell activity should be maintained as much as possible. The principle is that the yield is higher, the purity is better, and the inactivation is lower. Currently the most commonly used method is Ficoll density gradient centrifugation to directly separate and purify peripheral blood mononuclear cells. Its principle is mainly based on the difference in the specific gravity of various types of blood cell...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M1/00C12M1/38C12M1/36C12M1/34
Inventor 张秀敏李增山叶菁隋延仿曲萍黄杨曹云新吴雅岚林慧袁媛
Owner FOURTH MILITARY MEDICAL UNIVERSITY
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