Preparation method of Bifidobacterium infantis microcapsules
A technology of Bifidobacterium infantis and microcapsules, which can be used in microcapsules, food preparation, capsule delivery, etc., can solve problems that have not been applied, and achieve good acid resistance and enteric dissolution
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Embodiment 1
[0021] Mix the edible gelatin solution and the Bifidobacterium infantis suspension at a speed of 500 rpm, the ratio of the edible gelatin solution and the Bifidobacterium infantis suspension is 1:3, the concentration of the edible gelatin solution is 1.5%, add gum arabic solution, the concentration of gum arabic solution was 1.5%, after stirring at the same speed for 3 min, the ratio of gum arabic solution and edible gelatin solution was 1:1, and the concentration of bifidobacteria suspension was 14.29%. Use 10% acetic acid to adjust the pH of the mixture to 3.8-4.0, continue to stir for 20 min, and put the prepared solution in an ice bath to cool until the temperature is below 10 °C. Use 10% NaOH to adjust the pH of the mixture to 6.0, add transglutaminase at an enzyme concentration of 10 U / g gelatin, continue to stir for 3 min, treat at 10°C for 6 h, wash, filter, etc. The wet bifidobacterium infantis microcapsules can be obtained through the process.
[0022] observed unde...
Embodiment 2
[0024] Mix the edible gelatin solution and the Bifidobacterium infantis suspension at a speed of 400 rpm, the ratio of the edible gelatin solution and the Bifidobacterium infantis suspension is 1:3, the concentration of the edible gelatin solution is 2%, add gum arabic solution, the concentration of gum arabic solution was 2%, and after stirring for 3 min at the same speed, the ratio of gum arabic solution and edible gelatin solution was 1:1, and the concentration of bifidobacteria suspension was 14.29%. . Use 10% acetic acid to adjust the pH of the mixture to 3.8-4.0, continue to stir for 20 min, and put the prepared solution in an ice bath to cool until the temperature is below 10 °C. Use 10% NaOH to adjust the pH of the mixture to 6.5, add transglutaminase at an enzyme concentration of 10 U / g gelatin, continue stirring for 3 min, treat at 15°C for 6 h, wash, filter, etc. The wet bifidobacterium infantis microcapsules can be obtained through the process.
[0025] Take the ...
Embodiment 3
[0027] Take edible gelatin solution and Bifidobacterium infantis suspension and mix at 450rpm, the ratio of edible gelatin solution and Bifidobacterium infantis suspension is 1:3, the concentration of edible gelatin solution is 2%, add gum arabic solution , the concentration of gum arabic solution was 2%, after stirring for 3 min at the same speed, the ratio of gum arabic solution to edible gelatin solution was 1:1, and the concentration of bifidobacteria suspension was 14.29%. . Use 10% acetic acid to adjust the pH of the mixture to 3.8-4.0, continue to stir for 20 min, and put the prepared solution in an ice bath to cool until the temperature is below 10 °C. Use 10% NaOH to adjust the pH of the mixture to 6.0, add transglutaminase at an enzyme concentration of 15 U / g gelatin, continue to stir for 3 min, treat at 15°C for 8 h, wash, filter, etc. The wet bifidobacterium infantis microcapsules can be obtained through the process.
[0028] Take the suspension of newly prepared...
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