Solution for sperm detection
A technology of sperm and solution, applied in the direction of biological testing, material inspection products, etc., can solve the problems of large difference in penetration time and penetration distance of cervical mucus, large difference in physical and chemical properties of sperm nutrient solution, and cannot be effectively promoted.
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Embodiment 1
[0019] The research of embodiment 1 sperm detection solution
[0020] 1. Test materials
[0021] Semen Collection: Semen was obtained from fertile donors who abstained for 2-5 days, resulting from masturbation. According to the WHO manual, semen was concentrated on flash slides according to the revised stringent standard (91±72×10 6 / ml, range 5.0-271), total motility (55±16%, range 6.0-81) and normal sperm morphology (20±7.8%, range 1.0-42).
[0022] Collection of Cervical Mucus: Cervical mucus is obtained from many different women who spontaneously ovulate after artificial insemination. All samples were collected at 3-day intervals before ovulation. Mucus is drawn from the cervical canal into a polyethylene tube. After collection, cervical mucus should be evaluated, including determination of mucus filamentous, fern-like crystals, viscosity, and pH (WHO, 2001). Normal cervical mucus (pH>7.0) was divided into vials and stored at 4°C for use within 7 days. Only normally ...
Embodiment 2
[0043] Example 2 Research on sperm detection solution for patients with oligospermia / asthenospermia
[0044] 1. Test materials
[0045] Semen collection: Semen is from oligo / weak sperm patients, produced by masturbation after 2-5 days of abstinence.
[0046] Collection of Cervical Mucus: Cervical mucus is obtained from many different women who spontaneously ovulate after artificial insemination. All samples were collected at 3-day intervals before ovulation.
[0047] Preparation of native egg whites: Egg whites are sucked from around the yolk layer of fresh eggs with a syringe.
[0048] Preparation of hyaluronic acid solution (solution B) containing L-carnitine: within 60 minutes before the test, dissolve L-carnitine in Tyrode buffer and mix with hyaluronic acid solution to form a solution containing L-carnitine hyaluronic acid solution. The concentration of L-carnitine is 10-20mM / L.
[0049] Control migration medium: Tyrode's solution containing BSA (Commonwealth Serum L...
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