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Rapid and safe technique for performing pcr amplifications

An analysis technique, an iodination technique, is applied in the technical field of rapid and safe PCR amplification, and can solve problems such as increasing processing time

Inactive Publication Date: 2012-01-25
TRIOMED INNOVATIONS CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As such, researchers may need to conduct experiments in a Biosafety Level 3 or 4 (BSL-3 or BSL-4) laboratory, further increasing processing time

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0169] Triosyn T40 beads (0.025 g) were added to previously labeled tubes, 10 mL of VRE suspension (diluted with 300-500 μl for one colony) was added to the tubes, followed by vortexing the samples. Samples were analyzed for the presence of active VRE at time points of 0, 2, 5, 10, 15 and 30 minutes. It was found that at the 2 minute and 5 minute time points, the active VRE decreased, and by the 10 minute time point, there was essentially no active VRE in the tube. At the 15 min and 30 min time points, no active VRE was present. A control was set up during this experiment which consisted of vortexing the VRE suspension in the absence of Triosyn T40 beads, which resulted in no reduction in the amount of active VRE.

Embodiment 2

[0171] Triosyn T40 beads (0.025 g) were added to previously labeled tubes, 10 mL of VRE suspension (diluted with 300-500 μl for one colony) was added to the tubes, followed by vortexing the samples. Samples were analyzed for the presence of active VRE at time points of 0, 2, 5, 10, 15 and 30 minutes. It was found that at the 0 minute time point, the active VRE decreased and by the 2 minute time point, there was essentially no active VRE in the tube. At the 5, 10, 15 and 30 minute time points, no active VRE was present. A control was set up during this experiment which consisted of vortexing the VRE suspension in the absence of Triosyn T50 beads, which resulted in no reduction in the amount of active VRE.

Embodiment 3

[0173] Triosyn T40 beads (0.025 g) were added to previously labeled tubes, 10 mL of VRE suspension (diluted with 300-500 μl for one colony) was added to the tubes, followed by vortexing the samples. Samples were analyzed for the presence of active VRE at time points of 0, 2, 5, 10, 15 and 30 minutes. It was found that at the 0 and 2 minute time points, the active VRE decreased, and by the 5 minute time point, there was essentially no active VRE in the tube. At the 10, 15 and 30 minute time points, no active VRE was present. A control was set up during this experiment which consisted of vortexing the VRE suspension in the absence of Triosyn T45 beads, which resulted in no reduction in the amount of active VRE.

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PUM

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Abstract

This invention relates to methods for quick and safe identification of pathogens from biological samples. Iodinated resins may be employed to destroy a pathogen while leaving the pathogen's DNA in a state that can be analyzed. The DNA can then serve as a substrate for PCR analysis. The use of these iodinated resins work in a significantly quicker manner than prior art methods and allows scientists to spend a minimal time under Biosafety Level Three (BSL-3) conditions.

Description

field of invention [0001] The present invention relates to a novel broad-spectrum rapid preparation method for extracting DNA for use in PCR amplification reactions and other molecular biology methods. Background of the invention [0002] Polymerase chain reaction (PCR) provides a means to increase the copy number (amplification signal) of a sequence of interest with or without prior culturing of the organism, thus allowing increased detection in samples from mixed populations. Sensitivity of DNA sequences present in small amounts in samples with DNA (Ou, C.Y. et al., Science 239:292-295; Saiki, R.K. et al. Science 239:487-494; Saiki, R.K. et al. Science 230:1350-1354 ; Scharf, S.J. et al. Science 233: 1076-1078). The method involves melting the DNA and annealing short oligo primers to regions flanking the sequence of interest. In the presence of free deoxynucleotides, DNA polymerase is added to the mixture and DNA is extended from the primers across the region of interest...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/34A01N59/12G01N33/48
CPCC12Q1/6806G01N33/569
Inventor 皮埃尔·J·梅西尔马克·托德杰曼
Owner TRIOMED INNOVATIONS CORP