Sperm optimizing microflow control device and sperm optimizing method

Abstract

The invention discloses a sperm optimizing microflow control device which comprises a slide glass provided with a buffer liquid sampling pool, a sample detection pool and a sample collecting pool, and a support substrate for supporting the slide glass, wherein a buffer liquid connection channel is arranged between the buffer liquid sampling pool and the sample detection pool; a sample acting poolis arranged inside the sample detection pool; one or more screening channels are arranged between the sample detection pool and the sample acting pool, and at least one screening channel is arranged at one side on which the sample detection pool and the sample collecting pool are connected; and a sample collecting channel is arranged between the sample detection pool and the sample collecting pool. The invention also discloses a sperm optimizing method. According to the invention, sperms having high motility and high linear motion speed are naturally screened through simulating physiological state of interaction between semen and cervix uteri mucus in the sample acting pool and utilizing the glutinousness of the cervix uteri mucus, thereby avoiding the damage of centrifugation on the sperms. The device disclosed by the invention can be used as a tool for auxiliary reproduction technology.

Description

technical field [0001] The invention relates to a device for sperm selection, and also relates to a sperm selection method. Background technique [0002] Studies have shown that the incidence of infertility is increasing year by year. The average incidence of infertility in China is 7%-10%, of which male factors account for 30%. Sperm quality affects conception one of the key factors. Assisted reproductive technology offers a way to address this medical problem. [0003] However, the premise of implementing various assisted reproductive technologies is the need to obtain high-quality sperm from semen, and the need to separate white blood cells, epithelial cells, etc. in semen from high-quality sperm. The main factors for judging high-quality sperm are the motility of sperm, the linear motion speed of sperm, the normal morphology rate of sperm and the DNA damage rate of sperm. At present, commonly used sperm selection methods include upstream method and density gradient ce...

AI Technical Summary

Problems solved by technology

Although the upstream method and the density gradient centrifugation method can sort sperm with good vitality, they cannot simulate the sperm sorting process under physiological conditions, and the sorting process takes a long time and requires centrifugation. Excessive centrifugation steps are likely to generate oxygen free radicals , leading to peroxidative damage to sperm DNA, resulting in reduced sperm quality and affecting the success rate of assisted reproductive technology

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