Lactobacillus paracasei strain gmnl‑133, compositions for improving atopic dermatitis or other allergic diseases and uses thereof

A technology of atopic dermatitis and lactobacillus, applied in skin diseases, allergic diseases, drug combinations, etc., can solve the problems of atopic dermatitis symptoms that are difficult to eradicate, skin atrophy and thinning, acne, etc., and achieve improvement The effect of atopic dermatitis

Active Publication Date: 2017-07-07
GENMONT BIOTECH
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The traditional method of treating atopic dermatitis is topical steroid therapy, but the symptoms of atopic dermatitis are difficult to eradicate and often recur after stopping the drug
Furthermore, long-term use of steroid therapy is prone to side effects, such as skin atrophy and thinning, skin pigmentation changes, hair growth, acne, etc.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Lactobacillus paracasei strain gmnl‑133, compositions for improving atopic dermatitis or other allergic diseases and uses thereof
  • Lactobacillus paracasei strain gmnl‑133, compositions for improving atopic dermatitis or other allergic diseases and uses thereof
  • Lactobacillus paracasei strain gmnl‑133, compositions for improving atopic dermatitis or other allergic diseases and uses thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Embodiment 1, isolation, screening and identification of bacterial strains

[0030] The inventor isolated more than one hundred strains of Lactobacillus from healthy human gastrointestinal tract specimens, and established a strain bank of isolates. In order to find Lactobacillus that has the potential to slow down the treatment of allergy-related diseases, each Lactobacillus in the strain bank was co-cultured with mouse spleen cells, and the secretion of IFN-γ in mouse spleen cells was analyzed, and then the isolates were screened out. GMNL-133 is a lactobacillus that can stimulate mouse spleen cells to secrete large amounts of IFN-γ.

[0031] The isolate GMNL-133 was analyzed for identification of various strains. figure 1 It is the morphological figure of the isolate GMNL-133 under the microscope. The preliminary test results of the isolate GMNL-133 show that it is a Gram-positive bacillus without catalase, oxidase and motility, and can grow in both aerobic and anaero...

Embodiment 2

[0039] Embodiment 2, Lactobacillus paracasei strain GMNL-133 and the difference analysis of the Lactobacillus paracasei of other commercially available products

[0040] Mix Lactobacillus paracasei strain GMNL-133 with commercially available yogurt (such as Uni-President LP33 yogurt, which contains Lactobacillus paracasei strain LP33), yogurt (such as Genki yogurt from Japanese Kirin Company, which contains Lactobacillus paracasei strain KW3110 ), probiotic health food (such as Baisheng Biotech Weimin Probiotics, containing Lactobacillus paracasei strain BRAP-01) and other products containing Lactobacillus paracasei for differential analysis, including random amplified polymorphism DNA (RAPD) electrophoresis analysis and API50CHL identification system analysis.

[0041] RAPD electrophoresis analysis Utilizes the primer sequence 5'-CCGCGACGTT-3' (SEQ ID No: 2) to carry out polymerase chain reaction to analyze Lactobacillus paracasei strain GMNL-133 and Lactobacillus paracasei s...

Embodiment 3

[0051] Embodiment 3, Lactobacillus paracasei strain GMNL-133 and other Lactobacillus paracasei strains are in stimulating mouse spleen cells to produce IFN-γ secretion comparison

[0052] After the Lactobacillus paracasei strain GMNL-133 and the aforementioned Lactobacillus paracasei strains in various commercially available products, KW3110, LP33 and BRAP-01, were co-cultured with the spleen cells of normal mice, the spleen cells of the mice were compared. IFN-γ secretion.

[0053] RPMI 1640 medium containing 10% fetal bovine serum ( Gaithersburg, MD, USA) to culture spleen cells from BALB / c mice. Will 4x10 5 mouse spleen cells were added to a 96-well plate, and 10 6 Each of the above-mentioned Lactobacillus strains was cultured at 37° C. for 48 hours in an environment containing 5% carbon dioxide. Afterwards, the cell culture supernatant was treated with a commercially available ELISA kit (OptEIA Mouse IFN-γSet, BD Biosciences San Diego, CA, USA) to determine the IFN-...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to an isolated Lactobacillus paracasei strain GMNL‑133, which is preserved in the China Center for Type Culture Collection (CCTCC), and the preservation number is CCTCC No. M2011311. The Lactobacillus paracasei strain GMNL‑133 can be used to manufacture compositions for treating atopic dermatitis or other allergic diseases. In addition, the present invention relates to a composition comprising the Lactobacillus paracasei strain GMNL‑133, and the content of the Lactobacillus paracasei strain GMNL‑133 can effectively treat atopic dermatitis or other allergic diseases.

Description

technical field [0001] The present invention relates to a strain of Lactobacillus and its use, in particular to a strain of Lactobacillus paracasei and its composition for improving allergic diseases and its use. Background technique [0002] Allergy is an acquired immune adverse reaction to a normally harmless substance. Symptoms caused by allergic reactions include itching, coughing, wheezing, sneezing, teary eyes, inflammation and fatigue. Allergic reactions are thought to include an early specific immune response and a late inflammatory response. It has been reported that allergens (such as pollens and dust mites) mediate early allergic responses by stimulating high-affinity immunoglobulin E (IgE). Specifically, when mast cells and basophils are stimulated by allergens, they release histamine and cytokines. Cytokines then mediate the late allergic response by recruiting inflammatory cells. It has also been reported that the influx of eosinophils, macrophages, lymphoc...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & AuthorityPatents(China)
IPC IPC(8): C12N1/20A61K35/747A61P17/00A61P37/08A23L33/135C12R1/225
Inventor吕英震谢丰钦
OwnerGENMONT BIOTECH