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Application of Bacillus pumilus in control of stem rot of orchid

A technology of Bacillus pumilus and stem rot, applied in the directions of application, fungicides, chemicals for biological control, etc., can solve the problems such as Bacillus pumilus to control orchid stem rot that have not yet been found

Inactive Publication Date: 2013-12-18
ZHEJIANG UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] After retrieval, Chen Weiliang and others have used Bacillus pumilus to prevent and control rice sheath blight (see Chinese patent, publication number: CN101942403A, application number 201010201531.9), but no report has been found on the use of Bacillus pumilus to control orchid stem rot

Method used

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  • Application of Bacillus pumilus in control of stem rot of orchid
  • Application of Bacillus pumilus in control of stem rot of orchid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1. Preparation of wettable powder I:

[0035] The antagonistic bacterial fermentation broth prepared according to the invention patent "Bacillus pumilus and its cultivation method and use" with the application number 201010201531.9 was tested and the bacterial concentration reached 10 7 Above cfu / mL, mix the antagonistic bacteria fermentation broth with dry diatomaceous earth at a ratio of 1:1 (V / V) to prepare wettable powder I.

Embodiment 2

[0036] Example 2. Preparation of wettable powder II:

[0037] The crude antibacterial active substance extract prepared according to the invention patent "Bacillus pumilus and its cultivation method and use" with the application number 201010201531.9 is mixed with dry diatomaceous earth at a ratio of 1:1 (V / V) to make a wettable powder Ⅱ.

Embodiment 3

[0038] Example 3. The effect of wettable powder of Bacillus 223 strain (ie wettable powder I and wettable powder II) on orchid stem rot:

[0039] Wettable powder I and wettable powder II are respectively diluted with 200 weight times of water to obtain wettable powder I suspension and wettable powder II suspension respectively.

[0040] Take 90 clusters of orchids that have been diseased and have the same disease severity, and treat each cluster as follows:

[0041] Remove the bulbs and leaf bases that have obviously browned and rotted orchid seedlings;

[0042] The 30 clusters of orchids (clusters) after the above treatment are immersed in the wettable powder I suspension (subject to the immersion of the whole seedlings), and after standing for 30 minutes, the seedlings are transferred and cultivated in a new culture bowl.

[0043] The 30 clusters of orchids (clusters) after the above treatment are immersed in the wettable powder II suspension (subject to the immersion of the whole see...

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Abstract

The invention discloses application of Bacillus pumilus in control of stem rot of orchid. The Bacillus pumilus is named Bacillus pumilus 223 and collected in Wuhan University of China of China Center for Type Culture Collection on April 8, 2010 with the collection number of CCTCC NO:M2010075. Antagonistic bacteria fermentation liquid prepared from the Bacillus pumilus 223 CCTCC NO:M2010075 is mixed with kieselguhr to form wettable powder I; crude extract of an antibacterial active substance prepared from the Bacillus pumilus 223 CCTCC NO:M2010075 is mixed with kieselguhr to form wettable powder II; and the two kinds of wettable powder can be applied to controlling the stem rot of the orchid.

Description

Technical field [0001] The invention relates to the application of microorganisms in preventing and treating plant diseases, in particular to the application of Bacillus pumilus and its extracts in preventing and treating orchid stem rot. Background technique [0002] Orchid has been loved and treasured by people for thousands of years for its noble and elegant, delicate fragrance, unique flower shape, color and fragrance. At present, various places have vigorously developed orchids as high-profit precision agriculture projects. After several years of development, especially the orchid craze in the past few years, many farmers have made their fortunes with orchids. But on the other hand, with the large-scale planting of potted orchids and the domestication and promotion of wild orchids, the occurrence of their diseases has become more and more serious, and new diseases have appeared from time to time. Orchid stem rot is the most serious disease. [0003] The onset period of orchid...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N63/02A01P3/00
Inventor 陈卫良朱秋潮
Owner ZHEJIANG UNIV