Screening of new strain capable of producing camptothecin and method for preparing camptothecin

Abstract

The invention discloses a new strain capable of producing camptothecin and a method for preparing camptothecin by using the same. The new strain capable of producing the camptothecin-Phomopsis sp. B29 is conserved in China General Microbiological Culture Collection Center (CGMCC) on July 14, 2010, and has a conservation number of CGMCC No. 3988. The camptothecin is indole alkaloid with antitumor activity and is extracted from Camptotheca acuminate-nyssaceae plant of China, and the camptothecin has good curative effect on malignant tumors such as colon cancer, ovarian cancer and the like. Direct extraction of the camptothecin from the Camptotheca acuminate causes vegetation deterioration, ecological unbalance and other problems, and a chemical synthesis method has the disadvantages of long route, low yield and high cost; and aiming at the problems, endophytic fungi are separated and purified from the bark and fruit of the Camptotheca acuminate, and the strain capable of producing the camptothecin is screened from the endophytic fungi and then is used for preparing the camptothecin through fermentation. The new strain provided by the invention can be taken as a new medicine source for producing the camptothecin; and the method for preparing the camptothecin by fermenting the new strain is convenient to operate and has the advantage of low cost.

Description

technical field

[0001] The invention belongs to the field of separation, screening and microbial fermentation of plant endophytes, in particular to a method for isolating and purifying endophytes from camptotheca japonica, fermenting and screening camptothecin-producing strains, and preparing camptothecin from the screened strains. Background technique

[0002] Camptothecin is an indole alkaloid with anti-tumor activity extracted from Camptotheca acuminate by Wall et al. in 1966. At present, the main production method of camptothecin is to extract from camptothecin, and the content of camptothecin in camptothecin is very low, generally 0.01% to 0.1%, resulting in low extraction rate and high cost, and camptothecin is a national Level II focuses on the protection of wild plants, and cutting down a large number of camphor trees will easily lead to vegetation damage and ecological imbalance. The chemical total synthesis of camptothecin has been successful, but it cannot be put...

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