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Protecting liquid for freezing and storing human umbilical cord Wharton jelly tissue block

A technology of Wharton's glue tissue and human umbilical cord, applied in the field of cryopreservation protection solution, can solve problems such as high toxicity and side effects, residues, etc., and achieve the effects of less toxic and side effects, reduced toxicity and high quality

Inactive Publication Date: 2012-05-02
江苏省北科生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Under normal circumstances, people choose a single permeable cryoprotectant dimethyl sulfoxide to cryopreserve stem cells. After cryopreservation and recovery, there will be residual cryoprotectant solution. Clinically, stem cells with residual cryoprotectant dimethyl sulfoxide are used. , has high toxic and side effects, and can easily cause nausea, vomiting, abdominal pain and other adverse reactions

Method used

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  • Protecting liquid for freezing and storing human umbilical cord Wharton jelly tissue block
  • Protecting liquid for freezing and storing human umbilical cord Wharton jelly tissue block
  • Protecting liquid for freezing and storing human umbilical cord Wharton jelly tissue block

Examples

Experimental program
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Effect test

example 1

[0015] Take 500ml of culture medium DMEM / F12, add 0.1 mol of sucrose and mix well, filter it with a 0.22um membrane filter, pre-cool at 4°C, add 100ml of autologous cord blood serum pre-cooled at 4°C on an ice bath, and pre-cool at 4°C 0.8 mol of ethylene glycol, 0.4 mol of dimethyl sulfoxide pre-cooled at 4°C, and supplemented with culture medium DMEM / F12 to 1 L is the cryopreservation protection solution for human umbilical cord Wharton's jelly tissue blocks.

example 2

[0017] Take 500ml of culture medium DMEM / F12, add 0.3mol of sucrose and mix well, filter it with a filter of 0.22um filter membrane, pre-cool at 4°C, add 200ml of autologous cord blood serum pre-cooled at 4°C on the ice bath, and pre-cool at 4°C. 1.2 mol of cold ethylene glycol, 0.6 mol of dimethyl sulfoxide pre-cooled at 4°C, and supplemented with culture medium DMEM / F12 to 1 L is the cryopreservation protection solution for human umbilical cord Wharton's jelly tissue blocks.

example 3

[0019] Take 500ml of culture medium DMEM / F12, add 0.2mol of sucrose and mix well, filter it with a filter of 0.22um filter membrane, pre-cool at 4°C, add 150ml of autologous cord blood serum pre-cooled at 4°C on the ice bath, and pre-cool at 4°C. 1 mol of cold ethylene glycol, 0.5 mol of dimethyl sulfoxide pre-cooled at 4°C, and supplemented with culture medium DMEM / F12 to 1 L is the cryopreservation protection solution for human umbilical cord Wharton's jelly tissue pieces.

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Abstract

The invention discloses a protecting liquid for freezing and storing a human umbilical cord Wharton jelly tissue block. 1L of protecting liquid comprises 0.8 to 1.2mol of ethylene glycol, 0.4 to 0.6mol of dimethyl sulfoxide, 0.1 to 0.3mol of sucrose, 100 to 200mol of autologous cord blood serum and the balance of culture solution DMEM / F12. The autologous cord blood serum and the culture solution DMEM / F12 are used as basic liquids to provide nutrients for the tissue block, ensure osmotic equilibrium inside and outside cells, ensure that the environment of cells in the tissue block is similar to that in vivo, and ensure a good freezing and storing effect; the low-concentration ethylene glycol and dimethyl sulfoxide are used as osmotic freezing protective agents, so that the toxicity of the protecting liquid on the cells is reduced; and the sucrose is used as a non-osmotic freezing protective agent to increase the viscosity of the solution and relive osmotic pressure inside and outside cell membranes when the cells are frozen and stored. The protecting liquid is used for directly freezing and storing the human umbilical cord Wharton jelly tissue block, has a good freezing and storing effect, ensures small cell loss, and can provide high-quality umbilical mesenchymal stem cells for clinic.

Description

technical field [0001] The invention relates to a cryopreservation protection solution for human umbilical cord Wharton's jelly tissue blocks. Background technique [0002] The cells in human umbilical cord Wharton's Jelly (Wharton's Jelly) have the characteristics of stem cells such as self-renewal, multi-directional differentiation, and strong proliferation ability. We call them umbilical cord mesenchymal stem cells (huMSCs). Mesenchymal stem cells are an important member of the stem cell family. They are low-immunogenic stem cells. They have the characteristics of supporting hematopoiesis, promoting stem cell implantation, and regulating immunity. They are ideal seeds for repairing tissue and organ damage caused by aging and disease. cell. Umbilical cord mesenchymal stem cells are getting more and more attention because of their wide source, easy access, and all the characteristics of mesenchymal stem cells. More and more people start to store umbilical cord mesenchymal ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
Inventor 李伟杨兵欧阳溪宋云庆胡祥
Owner 江苏省北科生物科技有限公司
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