Mutant of endoglucanase, coding gene and application thereof

A technology of endoglucanase and coding gene, which is applied in the field of endoglucanase mutants, can solve problems such as low enzyme activity or yield, and cannot meet the needs of industrial production, and achieve a wide working temperature range, The effect of good industrial application prospects
CN102443576AActive Publication Date: 2012-05-09INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
Publication Date
2012-05-09

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Abstract

The invention discloses a mutant of endoglucanase, coding gene and application thereof. A protein provided by the invention is artificially synthesized and named as celA-mut(3F-6) or celA-mut(C3-13); the protein is a protein 1) or protein 2) as below: 1) a protein consisting of amino acid residue sequence in a sequence 2 in the sequence table; 2) a protein consisting of amino acid residue sequence in a sequence 4 in the sequence table; 3) a protein obtained by replacement and / or deletion and / or addition of one or a plurality of amino acid residues on the amino acid residue of the sequence2 or a sequence 4 in the sequence table, and with endoglucanase activity and derived from 1). Experiments of the invention have proved that the mutant of endoglucanase obtained by the invention has increased vitality compared with a wild type thereof and wide operational temperature range while maintaining an optimum reaction temperature (75 DEG C), therefore the mutant of endoglucanase has good industrial application prospect.
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Description

technical field

[0001] The invention relates to a mutant of endoglucanase, its coding gene and application. Background technique

[0002] Endoglucanase (1,4-β-D-endoglucanase, EC 3.2.1.4) can randomly act on the β-1,4-glycosidic bonds connecting glucose units in the long chain of cellulose from the inside to generate different lengths of glucose chains. If the enzyme works together with exoglucanase and β-glucosidase, the cellulose can be further degraded into glucose or other products that are easy to be further processed and converted, and finally generate high value-added biotechnology products. Therefore, endoglucanase has important application value in bioenergy, food processing, papermaking, washing and other industries.

[0003] Compared with other enzyme proteins, enzyme products derived from thermophilic bacteria not only have higher temperature stability, but also often have other stress resistance properties at the same time. Therefore, the application of therm...

Claims

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