Cleaning process for extracting and purifying tabersonine from voacango Africana stapf seeds

A Fukang tree seed and its Boring technology, applied in the field of clean technology, can solve the problems of high energy consumption for solvent recovery, cumbersome preparation steps, and low product yield, and achieve low cost, simple process, and high purity. Effect

Inactive Publication Date: 2014-12-10
成都合盛生物技术有限公司
View PDF5 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the process disclosed in the U.S. patent, a large amount of toxic solvents such as benzene and chloroform are used for extraction, and the preparation steps are cumbersome and the product yield is low
The process of CN 101250188A adopts organic solvent heating and reflux extraction, consumes a large amount of organic solvent, and recycles solvent consumes a large amount of energy, and because its boning changes during long-term heating, resulting in a low final yield of 1.6%

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Cleaning process for extracting and purifying tabersonine from voacango Africana stapf seeds

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] A cleaning process for extracting and purifying Taborin from the seeds of Fukang tree is carried out according to the following steps.

[0018] 1. Supercritical extraction: Dry 0.5 kg of Fukang tree seeds, crush them and pass through a 20-mesh sieve, put them into an extraction kettle, and extract them with supercritical carbon dioxide. The extraction temperature is 40°C, the extraction pressure is 30Mpa, the flow rate of carbon dioxide is 20L / h, and flow extraction is carried out after 20 minutes of equilibrium. Extract for 3 hours. The pressures of the analysis kettles A and B were respectively 8 and 6Mpa, and the temperatures were respectively 40 and 45°C. After analysis, 78mL of yellow oily liquid containing taborine was obtained.

[0019] 2. Preliminary separation: the yellow oily liquid was washed three times with 150mL of 0.5mol / L hydrochloric acid, combined with the acid and water, and adjusted to a pH value of about 10 with concentrated ammonia water, then ext...

Embodiment 2

[0022] A cleaning process for extracting and purifying Taborin from the seeds of Fukang tree is carried out according to the following steps.

[0023] 1. Supercritical extraction: Dry 0.5 kg of Fukang tree seeds, crush them and pass through a 40-mesh sieve, put them into an extraction kettle, and extract them with supercritical carbon dioxide. The extraction temperature is 45°C, the extraction pressure is 30Mpa, the flow rate of carbon dioxide is 20L / h, and flow extraction is carried out after 20 minutes of equilibrium. Extract for 4 hours. The pressures of the analysis kettles A and B were respectively 10 and 8Mpa, and the temperatures were respectively 45 and 50°C. After analysis, 76mL of yellow oily liquid containing taborin was obtained.

[0024] 2. Preliminary separation: the yellow oily liquid was washed three times with 150mL of 0.5mol / L hydrochloric acid, combined with the acid and water, and adjusted to a pH value of about 10 with concentrated ammonia water, then ext...

Embodiment 3

[0027] A cleaning process for extracting and purifying Taborin from the seeds of Fukang tree is carried out according to the following steps.

[0028] 1. Supercritical extraction: Dry 0.5 kg of Fukang tree seeds, crush them and pass through a 40-mesh sieve, put them into an extraction kettle, and extract them with supercritical carbon dioxide. The extraction temperature is 45°C, the extraction pressure is 30Mpa, the flow rate of carbon dioxide is 25L / h, and the flow extraction is performed after 20 minutes of equilibrium. Extract for 2 hours. The pressures of the analysis kettles A and B were respectively 10 and 8Mpa, and the temperatures were respectively 45 and 50°C. After analysis, 74mL of a yellow oily liquid containing taborin was obtained.

[0029] 2. Preliminary separation: the yellow oily liquid was washed three times with 150mL of 0.25mol / L hydrochloric acid, combined with the acid and water, adjusted the pH value to about 10 with concentrated ammonia water, then ext...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a cleaning process for extracting and purifying tabersonine from voacango Africana stapf seeds, belonging to the technical field of preparation of plant extracts. The process is used for extracting the tabersonine from the voacango Africana stapf seeds by adopting a carbon dioxide supercritical extraction technology; after initial separation of acid and alkali treatment and solvent extraction, the tabersonine is purified by using a column chromatography technology; and the obtained tabersonine has the purity of 98% and the yield of more than 2.0%. The cleaning process disclosed by the invention is a high-efficiency environment-friendly process which is high in sample yield, high in purify, simple and safe in process, good in controllability, low in cost and short in production cycle, and is applied to large-scale industrial production.

Description

Technical field: [0001] The invention relates to a cleaning process for extracting and purifying taborin from fukang tree seeds, and belongs to the technical field of preparation of plant extracts. Background technique: [0002] Voacango Africana Stapf, also known as African potato fruit, is the seed of Voacango Africana Stapf, a plant of the family Apocynaceae from Africa, and contains a variety of alkaloids. It Bo Ning (Tabersonine, alias: willow leaf water glycyrrhizin, CAS NO.4429-63-4, molecular formula C 21 h 24 N 2 o 2 , molecular weight 336.42) is the most important alkaloid component in the seeds of Fukang tree. It has antihypertensive, antitumor, hypoglycemic, and diuretic effects. It is used for depression, anxiety, and emotional instability caused by stroke sequelae, ischemic hypertensive encephalopathy, and cerebrovascular disease. It is suitable for premature aging cerebral palsy. The symptoms of degeneration are eliminated. Now it is mainly used as a sem...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Patents(China)
IPC IPC(8): C07D471/20
CPCY02P20/54
Inventor 邓赟刘海峰姚晨赵虎
Owner 成都合盛生物技术有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap