Primers and method for quickly distinguishing orange varieties

Abstract

The invention discloses primers and a method for quickly distinguishing orange varieties, and belongs to the field of molecular marking in molecular biology. The method comprises the steps of: designing RAPD (Random Amplified Polymorphic Deoxyribonucleic Acid) random primers according to the gene sequences of the orange, and screening to obtain 16 random primers; extracting DNA (Deoxyribonucleic Acid) of a plurality of orange varieties, and diluting for later use; and amplifying the DNA of the unknown orange varieties by utilizing the random primers one by one, distinguishing out the variety with unique characteristic band, and building a tree-shaped identification figure. According to the invention, 48 orange varieties can be distinguished on a molecular level through a plurality of PCR (Polymerase Chain Reaction), the practicability of RAPD molecular marking utilizing 11 base primers in plant variety identification is embodied to a certain extent, and the obtained tree-shaped identification figure is more illustrative than the clustering tree, namely the primers for distinguishing any two varieties can be found out according to the variety identification figure; and the method can realize early identification of orange seedlings, and has wide universality in other species.

Description

technical field [0001] The invention relates to the field of molecular biology molecular markers, in particular to a primer and a method for quickly distinguishing citrus varieties. Background technique [0002] Citrus belongs to Rutaceae fruit trees, including tangerine, tangerine, grapefruit, orange, lemon, etc., and is mainly distributed in the area south of 35° north latitude in the world. Citrus has always been one of the main fruit tree species planted in my country and the world. Citrus varieties are very rich in resources. Therefore, the establishment of a rapid and reliable identification technology system for Citrus species has important practical significance for early identification of seedlings, protection of species rights, differentiation of varieties in production, and research on the genetic relationship and genetic diversity of Citrus germplasm resources. So far, the traditional single morphological variety identification method is difficult to effectivel...

AI Technical Summary

Problems solved by technology

This patent uses a lot of primers, and the amount of operation in the quality identification is very large, and it is not time-sensitive. It is not easy to judge the primers to distinguish varieties through the clustering tree, and it is not intuitive enough.

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