Method and device for establishing bladder cancer in-situ model of nude mouse

A technology of in situ model and establishment method, which is applied in medical science, veterinary equipment, animal husbandry, etc., can solve the problems that in situ tumors are not suitable for establishment, human tumor cells are difficult to infiltrate, and do not conform to outward growth, etc., to achieve High cutting efficiency, short time and reduced workload
CN102499784AInactive Publication Date: 2012-06-20ๆŽไบ‘้พ™
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Patent Information

Authority / Receiving Office
CN ยท China
Patent Type
Applications(China)
Current Assignee / Owner
ๆŽไบ‘้พ™
Publication Date
2012-06-20
Estimated Expiration
Not applicable ยท inactive patent

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Abstract

The invention discloses a method for establishing a bladder cancer in-situ model of a nude mouse. The method is characterized by comprising the following steps of: narcotizing a female nude mouse superficially, and inserting a urethral catheter with a side hole into a bladder from a urethral canal, so that negative pressure is formed in the urethral catheter continuously; penetrating a cutter into the urethral catheter, and adjusting the positions of a cutting inclined plane and the side hole of the urethral catheter; cutting under the negative pressure to cut part of mucous membrane layers off, so that the lower layer of a bladder mucous membrane is exposed to form a local wound surface; and pouring human bladder cancer cell suspension which is in the logarithm growth period into a bladder cavity of the nude mouse and reserving for a period of time, discharging the cell suspension out, and establishing the superficial bladder cancer model after 7 days. Compared with the conventional electrocauterization method, the conventional chemical medicinal corrosion method and the conventional bladder puncture intracavity cutting method, the method has the advantages that molding assemblies are low in production cost, and the method for establishing the bladder cancer model is good in effect, the device is easy to operate, the time is short, the nude mouse has little pain, and death caused by anesthesia, operation strike and infection is reduced obviously.
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Description

technical field

[0001] The invention belongs to the establishment technology of animal models of human malignant tumors, and relates to the establishment of in situ models of nude mice for experiments. In particular, the invention relates to a method and device for establishing an orthotopic model of superficial bladder cancer in the human urinary bladder cavity. It can also be used for orthotopic implantation of human malignant tumor cells in the esophagus, colon, and uterus of nude mice. Background technique

[0002] Bladder cancer is the most common malignant tumor in the genitourinary system, and it has received extensive attention due to its high incidence and recurrence rate. Bladder cancer is mostly superficial when it first occurs. At present, the conventional treatment for superficial bladder cancer is transurethral resection, supplemented by infusion chemotherapy or infusion immunotherapy after surgery, but there is still a high recurrence rate of the tumor after ...

Claims

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