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Method and device for establishing bladder cancer in-situ model of nude mouse

A technology of in situ model and establishment method, which is applied in medical science, veterinary equipment, animal husbandry, etc., can solve the problems that in situ tumors are not suitable for establishment, human tumor cells are difficult to infiltrate, and do not conform to outward growth, etc., to achieve High cutting efficiency, short time and reduced workload

Inactive Publication Date: 2012-06-20
李云龙
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The deficiencies of the above-mentioned disclosed patent method: first, it also needs to surgically incise the abdominal cavity of the nude mouse after complete anesthesia to expose the organs to be inoculated and cover the tumor After the block, the organs are reset and the abdominal cavity is closed by suturing. The consequences are the same as those described above. Deep anesthesia and large surgical blows are required. If you are not careful, the nude mice will die. Antibiotics are injected after the operation to prevent infection and increase the risk of infection. Second, it is only suitable for the establishment of orthotopic tumors planted on the outer surface of organs, not suitable for the establishment of orthotopic tumors on the surface of the cavity
When the tumor mass is successfully planted, the outer solidified OB glue medical adhesive layer can prevent the tumor cells from growing outward, but can only infiltrate into the surrounding submucosa. Some people have done experiments on a variety of human tumor cells, and human tumor cells It is difficult to infiltrate and penetrate the serosa layer of the viscera of nude mice, which does not meet the characteristics of human tumors growing outward through the serosa layer and forming abdominal implants when the tumor tissue falls off

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  • Method and device for establishing bladder cancer in-situ model of nude mouse
  • Method and device for establishing bladder cancer in-situ model of nude mouse
  • Method and device for establishing bladder cancer in-situ model of nude mouse

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Embodiment Construction

[0045] The present invention will be further described below in conjunction with accompanying drawing:

[0046] like figure 1 , 2 , 3, the device of the present invention includes: suction syringe (1), syringe (2), blood transfusion hose (3), catheter with side hole through joint (4), catheter with side hole ( 5), cutter (7). The urinary catheter with a side hole is connected to the suction syringe through the joint (4) and the blood transfusion hose (3). The cutter (7) is a metal needle capable of passing through the blood transfusion rubber tube (3) and the joint (4) into the lumen of the urinary catheter to cut the bladder mucosa of the nude mouse at the suction side hole to form a local wound. The suction syringe can continuously form a negative pressure, and the distal end of the cutting needle is ground into an acute bevel with a sharp cutting edge, which can penetrate into the blood transfusion hose. The puncture hole can be tightly closed under negative pressure wit...

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Abstract

The invention discloses a method for establishing a bladder cancer in-situ model of a nude mouse. The method is characterized by comprising the following steps of: narcotizing a female nude mouse superficially, and inserting a urethral catheter with a side hole into a bladder from a urethral canal, so that negative pressure is formed in the urethral catheter continuously; penetrating a cutter into the urethral catheter, and adjusting the positions of a cutting inclined plane and the side hole of the urethral catheter; cutting under the negative pressure to cut part of mucous membrane layers off, so that the lower layer of a bladder mucous membrane is exposed to form a local wound surface; and pouring human bladder cancer cell suspension which is in the logarithm growth period into a bladder cavity of the nude mouse and reserving for a period of time, discharging the cell suspension out, and establishing the superficial bladder cancer model after 7 days. Compared with the conventional electrocauterization method, the conventional chemical medicinal corrosion method and the conventional bladder puncture intracavity cutting method, the method has the advantages that molding assemblies are low in production cost, and the method for establishing the bladder cancer model is good in effect, the device is easy to operate, the time is short, the nude mouse has little pain, and death caused by anesthesia, operation strike and infection is reduced obviously.

Description

technical field [0001] The invention belongs to the establishment technology of animal models of human malignant tumors, and relates to the establishment of in situ models of nude mice for experiments. In particular, the invention relates to a method and device for establishing an orthotopic model of superficial bladder cancer in the human urinary bladder cavity. It can also be used for orthotopic implantation of human malignant tumor cells in the esophagus, colon, and uterus of nude mice. Background technique [0002] Bladder cancer is the most common malignant tumor in the genitourinary system, and it has received extensive attention due to its high incidence and recurrence rate. Bladder cancer is mostly superficial when it first occurs. At present, the conventional treatment for superficial bladder cancer is transurethral resection, supplemented by infusion chemotherapy or infusion immunotherapy after surgery, but there is still a high recurrence rate of the tumor after ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61D7/00A01K67/027
Inventor 李云龙
Owner 李云龙
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