Water phase dispersing method for lyophobic organic dying nanoparticles

Abstract

The invention discloses a water phase dispersing method for lyophobic organic dying nanoparticles. The method comprises the following steps of: (1) preparing lyophobic organic fluorescent dying molecules into precursor nanoparticles with a solvent exchange method, (2) preparing an amphipathic surfactant into 1mg/mL amphipathic surfactant aqueous solution; and (3) adding 300 muL of 1 mg/mL amphipathic surfactant aqueous solution into a suspension of the precursor nanoparticles, performing ultrasonic dispersion for 5 minutes, and standing for 6 hours to obtain water phase-dispersed lyophobic organic dying nanoparticles. In the method, surface modification of the lyophobic organic dying nanoparticles is realized by controlling the type and concentration of the amphipathic surfactant. The preparation method is easy and practicable, and can be widely applied in the fields of biology, medicine, chemistry and the like; and an experimental basis is provided for the preparation of other water phase-dispersed lyophobic organic small molecular fluorescent nanoparticles.

Description

technical field [0001] The invention belongs to an aqueous phase dispersion method of hydrophobic organic dye nanoparticles, in particular, the invention relates to the preparation of hydrophobic organic dye nanoparticles and the surface hydrophilic modification of nanoparticles. [0002] Background technique [0003] Organic dyes are widely used in the field of biological imaging. However, they face many problems, such as easy aggregation of hydrophobic organic dyes in aqueous solution, low photostability, etc., which greatly limit their application. Therefore, in recent years, a large number of improved bioluminescent probes based on organic dyes have emerged, mainly including: composite silicon sphere structures formed by dyes connected to silicon substrates through physical adsorption or chemical bonds; dye-doped polymer nanostructures . In these systems, since the direct contact between the dye and the external chemical environment is avoided, the photobleaching resis...

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Problems solved by technology

[0004] In addition, although the inorganic quantum dot fluorescent probes developed in recent years have the advantages of high brightness and high photostability, the problem of their own potential toxicity has been unavoidable (Hoshino, A.; Fujioka, K.; Oku, T .; Suga, M.; Sasaki, Y. F.; Ohta, T.; Yasuhara, M.; Suzuki, K.; Yamamoto, K. Nano Lett. 2004, 4, 2163-2169)