High-speed structure illumination optical microscope system and method based on digital micromirror device

A digital micromirror device and structured lighting technology, applied in optics, microscopes, optical components, etc., can solve the problems of slow speed and low utilization rate of microscopic light energy, and achieve the elimination of image quality. The effect of high utilization of light energy

Inactive Publication Date: 2012-07-04
XI'AN INST OF OPTICS & FINE MECHANICS - CHINESE ACAD OF SCI
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Problems solved by technology

[0012] Aiming at the technical problems of low light energy utilization rate and slow speed in the current structured lighting, the present invention

Method used

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  • High-speed structure illumination optical microscope system and method based on digital micromirror device
  • High-speed structure illumination optical microscope system and method based on digital micromirror device
  • High-speed structure illumination optical microscope system and method based on digital micromirror device

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Example Embodiment

[0050] Such as figure 1 As shown, a high-speed structured lighting optical microscopy system based on digital micromirror devices includes an illuminating light source 1, a beam splitter prism 2 arranged on the light path of the illuminating light source 2, a structured light generator 3 arranged on the reflection light path of the light splitting prism 2 The lens 4 arranged on the transmission light path of the beam splitting prism 2, the beam splitter 5 arranged on the optical path of the lens, the microscope objective lens 6 and the stage 7 arranged on the optical path above the beam splitter 5, the light arranged below the beam splitter 5 The mirror 8 and tube lens 9 on the road, and the CCD camera 10 arranged behind the tube lens.

[0051] Generally, the structured light generator 3 is a digital micromirror device DMD. Illumination light source can choose monochromatic or multi-color LED light source according to different samples

[0052] The invention has two working modes:...

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Abstract

The invention relates to a high-speed structure illumination optical microscope system and a method based on a digital micromirror device. The high-speed structure illumination optical microscope system comprises an illumination light source, a light splitting prism, a structure light generator, a lens, a spectroscope, a microobjective, an objective table, a reflector, a tubular mirror and a charge coupled device (CCD) camera, wherein the light splitting prism is arranged on a light path of the illumination light source, the structure light generator is arranged on a reflection light path of the light splitting prism, the lens is arranged on a transmission light path of the light splitting prism, the spectroscope is arranged on a lens light path, the microobjective and the objective table are arranged on a light path above the tubular mirror, the reflector and the tubular mirror are arranged on a light path under the spectroscope, and the CCD camera is arranged behind the tubular mirror. The system and the method aim at the technical problems of low optical energy utilization rate and low speed of the existing structure illumination microscope and have the advantages that the image refreshing speed is high (32KHz), and the optical energy utilization rate is high (higher than 90 percent). The system and the method are more applicable to the real-time three-dimension image study and high-speed dynamic process observation of living biological cells.

Description

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Claims

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Application Information

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Owner XI'AN INST OF OPTICS & FINE MECHANICS - CHINESE ACAD OF SCI
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