Screening and application of Bohai Bay acremonium cephalosporium

A technology of marine fungus, Acremonium acremonium, which is applied in the direction of nematicides, applications, fungicides, etc., and can solve the problem of lack of strain resources for efficient production of nematicides

Inactive Publication Date: 2012-07-11
TIANJIN NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] An object of the present invention is to overcome the deficiencies in the existing nematicides and the shortage of strain resources for high-efficiency production of nematicides, so as to provide a nematicidal filamentous marine fungus Acremonium acremonium in Bohai Bay with nematicidal function Strain BH-0531 (Accession No. CGMCC-5445)

Method used

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  • Screening and application of Bohai Bay acremonium cephalosporium
  • Screening and application of Bohai Bay acremonium cephalosporium
  • Screening and application of Bohai Bay acremonium cephalosporium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0117] Screening of Acremonium acremonium BH-0531 strain

[0118] (1) Separation and purification of strains

[0119] The bottom seawater sample collected from the Bohai Sea (longitude 117°53' 23.2", latitude 38°55' 51.0") was filtered through a 0.45 μm nitrocellulose filter membrane (diameter 50, commercially available), and the filter membrane was placed in seawater solid culture medium at a constant temperature of 26°C. Adopt single spore and transfer it to a new plate for culture. This process is repeated until the colonies are purified. The seawater solid medium: glucose 10g / L, peptone 2g / L, yeast extract 2g / L, seawater 600mL / L, distilled water 400mL / L, agar 20g / L.

[0120] (2) Screening of strains:

[0121] 1) Inoculate the above-mentioned isolated and purified strains into liquid medium for shake flask fermentation culture, and the culture liquid after fermentation culture is filtered through a filter membrane (commercially available) with a pore size of 0.22 μm,...

Embodiment 2

[0130] Cultivation of Acremonium strain BH-0531 (CGMCC-5445):

[0131] The best medium is: 150g / L peeled potato, 10g / L glucose, 2g / L yeast extract, 600ml / L seawater; the 150g / L peeled potato refers to: 150g peeled potato placed in 400mL Boil the juice in distilled water, take the juice, and then dilute to 400mL;

[0132] The optimal culture conditions are: spore suspension with an inoculum size of 2×106, a rotational speed of 120r / min, an initial pH of 6.5, and fermentation at 26°C for 96h.

Embodiment 3

[0134] Acremonium strain BH-0531 (CGMCC-5445) has a wide range of nematodecidal conditions and wide applicability:

[0135]The fermentation filtrate of the BH-0531 strain still retains good nematocide activity after being treated at 121°C for 20 minutes, and its activity remains above 95% of the original activity. The applicable pH of the fermentation filtrate is also relatively wide (see Table 7), and the fermentation metabolites have good water solubility (Table 8). Since the soil in my country is generally acidic in the south and alkaline in the north, the nematicide active substance produced by Acremonium acremonium strain BH-0531 has more advantages in implementation and application, and has overcome the problem that most of the existing nematicides are organic-soluble and water-soluble. Disadvantages of poor sex.

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Abstract

The invention provides an acremonium strictum strain BH-0531 separated from a Bohai Bay water area and screening and an application thereof. The strain is collected in the China General Microbiological Culture Collection Center with the collection number of CGMCC No.5445. The strain is separated from a sample of bottom layer seawater in the Bohai Bay, and is obtained by screening and identifying.The BH-0531 strain separated and screened in the invention belongs to acremonium strictum (originally called cephalosporium), has the characteristics of low growing temperatures of marine microorganisms and growth under high-salt conditions, and has the capability of producing nematicidal active metabolic products; produced nematicidal substances have high thermal stability and water solubility; the nematicidal activity can be over 94 percent under experimental conditions; and the acremonium strictum strain BH-0531 can be applied to fermentation production of bioactive substances such as nematicides, antibiotics and the like.

Description

technical field [0001] The invention belongs to marine biological resources and microbial strains, and is embodied in a nematicide-killing Acremonium strain ( Acremonium cephalosporium ) BH-0531 and its screening and application. Background technique [0002] As an important class of pathogens, nematodes have become an important problem in agricultural and forestry production. There are more than 200 genera and 5,000 species of plant parasitic nematodes reported in the world. Plant parasitic nematode diseases bring economic losses of nearly 125 billion US dollars to agriculture worldwide every year, second only to fungal diseases. Therefore, the management of soil and plant parasitic nematodes has become a global and urgently important problem to be solved. Chemical nematicides, such as methyl bromide, which appeared in the early 1940s, and carbamates and organic phosphates, which appeared in the late 1950s, are mostly toxic pesticides and have been found to have teratoge...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12N1/02C12Q1/04A01N63/04A01P5/00A01P3/00A01P1/00C12P1/02C12R1/645
Inventor 孟庆恒史晓讯孟凡欢冯欣孙建华孙金生
Owner TIANJIN NORMAL UNIVERSITY
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