Composite biological agent and preparation method thereof

A technology of biological agents and physiologically active substances, which is applied in the field of compound biological agents containing seaweed physiologically active substances and rhizosphere growth-promoting bacteria and its preparation, can solve the problem of organic carbon conversion and nutrient supply capacity decline, soil biochemical process intensity problems such as weakening, biological population and functional diversity attenuation, to enhance the effect of increasing production and saving fertilizer, eliminating the influence of soil microorganisms, and reducing environmental pollution

Active Publication Date: 2012-07-18
SHANDONG BAOYUAN BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Under the traditional agricultural ecosystem, the large-scale application and high-intensity use of chemical fertilizers lead to the reduction of soil microbial biomass, the decline of biologi

Method used

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  • Composite biological agent and preparation method thereof
  • Composite biological agent and preparation method thereof
  • Composite biological agent and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Embodiment 1 cover bubble treatment process:

[0048] (1) Wash the collected kelp to remove sundries, weigh 200kg, and break the kelp below 2cm in diameter;

[0049] (2) First put 100kg of algae dregs in pool A to soak for 60 minutes, after soaking, remove the algae dregs and put them in pool B for another 60 min. Add 500kg of tap water to pool A, and remove the algae residue after soaking in pools A and B twice; at the beginning of the second round, the order of pools A and B is exchanged. At this time, pool A is clean fresh water, and pool B is The soaking liquid after the second soaking of the algae dregs, the new 100kg algae dregs are put into the B pool for soaking for 60 minutes, after soaking, the algae dregs are taken out and put into the A pool for another 60 min, and the soaking solution in the B pool is put into Add 500kg of tap water to pool B again in the container to be treated, and after soaking in pools B and A twice, remove the algae residue for the ne...

Embodiment 2

[0050] Embodiment 2 The steps of rhizosphere growth-promoting bacteria colonization and domestication and high-density cultivation:

[0051] (1) Four rhizosphere-promoting bacteria (Enterobacter cloacae 10014, Bacillus licheniformis 23584, Pseudomonas aeruginosa, Bacillus Mucilaginosus) were used. Mix and inoculate in 50mL culture solution, the inoculation amount of each bacteria is 10 6 / mL, put a piece of filter paper at the bottom of the sterilized Petri dish, pour 50mL of the above-treated culture solution into it, then put 50 small rapeseed seeds, cover the top with a piece of wet filter paper, and then put it in the sterile room Cultivate and accelerate germination at 30°C; remove the upper layer of filter paper after 3 days and continue to cultivate.

[0052] (2) Cultivate for another 3 days, wash the mixed bacteria on the root tips of the seedlings with the culture solution, and then inoculate them on fresh seeds for the next round of enrichment cycle (the operation i...

Embodiment 3

[0054] The preparation method 1 of embodiment 3 composite preparations

[0055] (1) Bubble treatment:

[0056] Rinse the collected kelp to remove debris, weigh 200kg, and crush the kelp to a diameter of less than 2cm; put 100kg of algae residue in pool A and soak for 60 minutes, after soaking, remove the algae residue and put it in pool B for another 60 minutes. At the same time, the soaking solution in pool A was evaporated and concentrated at 50°C, and 500kg of tap water was added to pool A, and the algae residue was removed after two soaks in pools A and B; at the beginning of the second round, the order of pools A and B was interchanged At this time, pool A is clean fresh water, and pool B is the soaking liquid after the second soaking of algae residues. The remaining 100kg of algae residues are first soaked in pool B for 60 minutes. After soaking, remove the algae residues and put them in pool A. Soak for another 60 minutes, and at the same time, evaporate and concentrat...

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Abstract

The invention relates to a composite biological agent and a preparation method thereof. The composite biological agent is mixed by seaweed physiological activator extracting solution and rhizosphere promotion bacterium liquid according to the volume ratio of (1-20):100. The composite biological agent contains a plurality of physiological activators and rhizosphere promotion flora, and has a plurality of advantages of resisting disease, moving soil, being free of public nuisance and the like.

Description

technical field [0001] The invention relates to the field of biological technology, in particular to a composite biological preparation containing seaweed physiologically active substances and rhizosphere growth-promoting bacteria and a preparation method thereof. Background technique [0002] Seaweed is a low-level cryptogamous plant that grows in the ocean. Due to its special growth environment, its nutritional composition is very different from that of terrestrial plants. It contains rich mineral elements and vitamins that cannot be compared with general terrestrial plants. Among them A variety of ingredients such as amino acids, minerals, polysaccharides, vitamins and physiologically active substances such as cytokinins, auxins and abscisic acid can establish a fertilization system similar to a cocktail, increase the vascular bundle cells of plant stems, and help plants establish Robust and powerful root system can promote the absorption and utilization of soil nutrients...

Claims

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Application Information

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IPC IPC(8): C05F11/08A01N65/03A01N63/02A01P21/00A01P1/00
Inventor 苏群
Owner SHANDONG BAOYUAN BIOTECH CO LTD
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