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Method for preparing porous chitosan scaffold

A technology of porous chitosan and chitosan, which is applied in the field of preparation of porous chitosan scaffolds, can solve the problem that the plasticity and microstructure connectivity of chitosan scaffolds cannot meet the requirements of the research field of cell culture technology, and the preparation method is only Limits and other issues, to achieve the effect of easy molding, good connectivity, and increased connectivity

Inactive Publication Date: 2012-07-18
UNIV OF SHANGHAI FOR SCI & TECH
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  • Abstract
  • Description
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Problems solved by technology

However, its preparation method is often limited to the commonly used freeze-drying method
The chitosan scaffold prepared by the traditional freeze-drying method is difficult to meet the requirements of the technical research field of cell culture in terms of plasticity, microstructure and connectivity between pores.

Method used

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  • Method for preparing porous chitosan scaffold
  • Method for preparing porous chitosan scaffold

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Embodiment 1

[0043] see figure 1 , the invention discloses a preparation method of a porous chitosan scaffold, first prepare a chitosan acid solution dissolved with a set amount of ammonium chloride, inject the solution into a mold for the first freeze-drying, and then freeze-dry The final stent is soaked in sodium hydroxide solution for a set time, the stent is washed with distilled water, and then the stent is freeze-dried for the second time to obtain the porous chitosan stent. Specifically, the preparation method includes the following steps:

[0044] (1), the preparation step of solution

[0045] ①. Prepare chitosan acid solution dissolved with a certain amount of ammonium chloride

[0046] First measure 1ml of acetic acid and add it to a 100ml volumetric flask to make a constant volume to prepare a 1% (V / V, volume ratio) acetic acid solution, weigh 0.2g of ammonium chloride, add it to the 1% (V / V) acetic acid solution and stir evenly Finally, take by weighing 3.0g chitosan again, ...

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Abstract

The invention discloses a method for preparing a porous chitosan scaffold. The method comprises the following steps of: preparing an acid solution of chitosan in which a set amount of ammonium chloride is dissolved; injecting the solution into a mold for primary freeze drying; soaking the scaffold subjected to freeze drying in a sodium hydroxide solution for set time, and cleaning the scaffold with distilled water; and performing secondary freeze drying on the scaffold to obtain the porous chitosan scaffold. By the method for preparing the porous chitosan scaffold, the porosity and the communication between holes of the chitosan scaffold can be increased.

Description

technical field [0001] The invention belongs to the technical field of preparation of porous materials, and relates to a porous chitosan support, in particular to a preparation method of the porous chitosan support. Background technique [0002] At present, whether it is laboratory or industrial production, the cell culture microcarriers used are mainly solid Cytodex series carriers, which belong to the monolayer monolayer culture method. In vivo, the growth and development process of tissues and cells is carried out under three-dimensional internal environment conditions, and the mutual signal transmission between cells and cell matrix and cells has an important impact on the regulation of cell proliferation and differentiation. Conventional in vitro single The multilayer culture method cannot provide the three-dimensional environmental conditions required for the normal growth and development of tissues, so the preparation of three-dimensional porous scaffolds is of great ...

Claims

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Application Information

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IPC IPC(8): C08J9/26C08J9/36C08L5/08C12M3/00
Inventor 李琳刘宝林韩宝三李维杰
Owner UNIV OF SHANGHAI FOR SCI & TECH
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