Radish cytoplasmic male sterility molecular markers and method for assisting selective breeding using same

Abstract

The invention discloses radish cytoplasmic male sterility molecular markers and a method for assisting selective breeding using same. The method comprises the steps of: based on genome DNA (Deoxyribonucleic Acid) of fertility separated F2 generation and first backcross generation of white radish cytoplasmic sterility material as templates, carrying out RAPD (Random Amplified Polymorphic DNA) analysis and multiple repeated tests to screen to obtain two RAPD markers, namely S88-1526 and S1344-415, linked with the radish cytoplasmic sterility fertility restorer gene, wherein the S88-1526 and S1344-415 markers are respectively located at both sides of the restorer gene and have the genetic distances of 4.2cm and 6.9cm, respectively. Furthermore, the RAPD marker S88-1526 is successfully transformed into SCAR (Sequence Characterized Amplified Region) marker scar-88. Linkage analysis shows that the cross-over value between the marker scar-88 and the restorer gene is 4.28%, and the corresponding genetic distance is 4.24cm. The radish cytoplasmic male sterility molecular markers can be used for performing assisted selection of early molecular markers during radish cytoplasmic male sterility breeding, so as to accelerate the breeding progress.

Description

technical field [0001] The invention belongs to the fields of agricultural vegetable variety selection and biotechnology, and in particular relates to RAPD and SCAR markers of radish cytoplasmic male sterility and their application for auxiliary selection breeding. Background technique [0002] For more than 30 years, plant male sterility has been a hot field in crop genetics, breeding and biological research. Cytoplasmic male sterility (cytoplasmic male sterility, CMS) is one of the most useful types of male sterility. In the 1970s, Ogura reported that cytoplasmic male sterility (CMS) in radish was jointly controlled by cytoplasmic sterile gene S and two pairs of nuclear sterile genes ms. Since the discovery of Ogura sterile cytoplasm, scholars at home and abroad have conducted various researches on the sterility mechanism and restoration genes of radish male sterility. slow. RAPD (Random Amplified Polymorphic DNA) was proposed by two research groups of Williams and Wels...

AI Technical Summary

Problems solved by technology

Due to sterility, the traditional breeding method needs to wait until the flowering stage is completed through direct observation in the field. For the excellent materials carrying the restorer gene, in order to transfer them into a sterile line, they must go through crossing, selfing, and test crossing with the sterile line at the same time. The process of judging its genotype is time-consuming and labor-intensive, the cycle is long, and the breeding process is slow

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