Method for regulating and controlling vitellogenin level of zebra fishes

Abstract

The invention discloses a method for regulating and controlling a vitellogenin level of zebra fishes. The method comprises the steps of: quantifying content of vitellogenin in blood plasma, whole body homogenate or head-tail homogenate of an aquatic organism to be detected; when the content of the vitellogenin in the blood plasma, the whole body homogenate or the head-tail homogenate of the aquatic organism to be detected is higher than the content of the vitellogenin in the blood plasma, the whole body homogenate or the head-tail homogenate of a reference aquatic organism, indicating that the aquatic organism to be detected is poisoned or interfered by a low-concentration estrogen interferent. The method disclosed by the invention has the advantages of being capable of detecting whether the aquatic organism is poisoned or interfered by median- and low-concentration estrogen interferents in an exogenous environment and having important implications for developing relevant scientific research, environment pollutant supervisory control, safety management and the like.

Description

technical field [0001] The invention relates to a method for evaluating the toxicity or interference of organic pollutants to the endocrine of aquatic organisms, in particular to a method for testing whether the endocrine of aquatic organisms is poisoned or interfered by low-concentration estrogen-like disruptors in the exogenous environment, belonging to The field of inspection of estrogen-like disruptors. Background technique [0002] The chemical pollutants in the ecological environment interfere with the endocrine function of humans and wild animals, thereby exerting various effects on individual reproduction, development, and sexual behavior, and even affecting the survival and reproduction of humans (Tilghman S L, Nierth-Simpson E N, Wallace R, et al. Environmental hormones: Multiple pathways for response may lead to multiple disease outcomes [J]. Steroids, 2010, 75(8-9): 520-523). Therefore, international organizations and developed countries implement mandatory mana...

AI Technical Summary

Problems solved by technology

When aquatic organisms are poisoned or polluted by low concentrations of estrogen-like disruptors, neither the external morphological characteristics nor the internal physiological indicators will change significantly; i

Images