Method for measuring growth cycle of puccinia striiformis by using microcalorimetric method

A technology of growth cycle and microcalorimetry, which is applied in the field of plant disease monitoring and research, can solve the problems of low accuracy, complicated and cumbersome operation, and large selection of culture medium, and achieve the effect of simple operation and high reliability

Inactive Publication Date: 2012-08-22
XIAN UNIV OF SCI & TECH
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Problems solved by technology

[0003] At present, the determination of the growth cycle of wheat stripe rust in this field mainly adopts counting method or photoelectric turbidimetry: counting method includes dilution plate counting method and microscope direct counting method, which is to inoculate a small amount of purebred single-cell wheat stripe rust to a quantitative In the liquid medium, samples are taken at regular intervals to measure the number of cells, and then the curve is drawn. Although this method is simple in principle, the operation is complicated and cumbersome, and the accuracy is not high. It is an automatic control method based on the principle that luminosity is inversely proportional, but this method can only detect suspensions containing a large number of bacteria, and has certain limitations for samples that are too dark in color, resulting in the selectivity of its determination to the culture medium bigger

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  • Method for measuring growth cycle of puccinia striiformis by using microcalorimetric method
  • Method for measuring growth cycle of puccinia striiformis by using microcalorimetric method
  • Method for measuring growth cycle of puccinia striiformis by using microcalorimetric method

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Embodiment Construction

[0015] The content of the present invention will be further described below in conjunction with an embodiment.

[0016] 1. Preparation of strains—according to the typical wheat stripe rust leaves collected in the field, the strains of wheat stripe rust were obtained by the shaking method, and the concentration of the prepared strains was 0.08g / l;

[0017] 2. Preparation of nutrient solution—get 2% by weight of glucose solution and 98% by weight of distilled water to prepare 500ml of wheat stripe rust germination nutrient solution, and the pH of the nutrient solution is 6 to 7;

[0018] 3. Pipette 10ml of culture solution into two test tubes A and B respectively, then add 0.8mg of wheat stripe rust to A, and use the culture solution in B as a reference solution. The temperature of the culture solution is 10°C to 15°C. Place test tubes A and B in a shaker for 24 hours;

[0019] 4. At normal pressure and at a selected temperature of 28°C, pipette 3ml of the solution from test tu...

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Abstract

The invention relates to a method for measuring the growth cycle of puccinia striiformis by using a microcalorimetric method. The method comprises the following steps: under normal pressure and selected temperature, measuring the heat flow value of culture solution of puccinia striiformis in real time by using a C80 microcalorimetric instrument, storing the heat flow value in a computer, and then calculating to obtain a growth cycle curve of puccinia striiformis. The method has the advantages of being simple and convenient in operation as well as high in reliability. According to the measured growth cycle curve, the growth stages, the time length in the growth stages, the heat varying speed and the specific value in the growth cycle of puccinia striiformis can be clearly distinguished so as to provide useful reference for researching and preventing puccinia striiformis.

Description

technical field [0001] The invention belongs to the technical field of plant disease monitoring and research, and relates to a method for measuring the growth period of wheat stripe rust fungus by microcalorimetry. Background technique [0002] Wheat stripe rust is an important worldwide wheat disease caused by Puccinia striiformis West.f.sp.tritici. It mainly occurs in winter wheat regions and northwest spring wheat regions in Northwest, Southwest, North and Huaibei regions of China. The disease has the characteristics of wide distribution, fast spread, and large damage area. Generally, it can reduce wheat production by 20% to 30% in popular years, and it can reduce wheat production by 60% to 90% in pandemic years, or even no harvest. The research on the growth of rust fungus is also a major issue related to the people's livelihood of the country. [0003] At present, the determination of the growth period of wheat stripe rust in this field mainly adopts counting method or...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N25/20
Inventor 刘向荣赵顺省宋超超王君蔡会武李侃社
Owner XIAN UNIV OF SCI & TECH
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