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Rapid propagation method for Pinellia ternate buds

A technology of seed buds and pinellia, which is applied in the field of rapid propagation of pinellia tubers, a traditional Chinese medicinal material, can solve the problems of complicated tissue culture rapid propagation procedures, high production investment costs, and high requirements for operators, and achieve low cost, low risk, and professionalism. Technically demanding effects

Inactive Publication Date: 2012-09-19
江西喜悦生物医药科研中心有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a technique for rapid propagation of Pinellia zizi Buds, and to propagate Pinellia Pinellia Buds in large quantities as seed stems, so as to overcome the low natural reproduction coefficient of Pinellia Pinellia, complicated procedures for tissue culture rapid propagation, high requirements for operators, and high production investment costs. Disadvantages, alleviating the shortage of provenance of pinellia

Method used

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  • Rapid propagation method for Pinellia ternate buds
  • Rapid propagation method for Pinellia ternate buds
  • Rapid propagation method for Pinellia ternate buds

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] (1) Select healthy and disease-free Pinellia tuber parent bodies with a diameter greater than 1 cm, remove mud block impurities, wash, and dry naturally;

[0026] (2) Disinfect the cutting knife and the operating table with 75% alcohol;

[0027] (3) Carry out wound treatment to Pinellia tuber parent body with the sterilized cutting knife: the Pinellia tuber parent body is equidistantly cross-cut twice into terminal bud part, middle part and lower part;

[0028] (4) the pinellia tuber parent terminal bud portion of step (3) is longitudinally cut into several fritters, and the fritters are directly placed in the soilless matrix vermiculite, cultivated under 2000lux light, and the cultivation time is 12h / d, the culture temperature is 26±1°C, and the small pieces of the terminal buds germinate and grow to complete small tubers;

[0029] (5) The obtained complete tubers with buds are directly used as provenance to supply Pinellia pinellia for field planting.

Embodiment 2

[0031] (1) Select healthy and disease-free Pinellia tuber parent bodies with a diameter greater than 1 cm, remove mud block impurities, wash, and dry naturally;

[0032] (2) Disinfect the cutting knife and the operating table with 75% alcohol;

[0033] (3) Carry out wound treatment to Pinellia tuber parent body with the sterilized cutting knife: the Pinellia tuber parent body is equidistantly cross-cut twice into terminal bud part, middle part and lower part;

[0034] (4) Cutting the Pinellia tuber parent body of step (3) into the middle and lower parts, and placing it on the peat of the moisture-retaining substrate, preferably with the naturally upward growth side of Pinellia, cultivating it under 2000lux light, and the light time is 12h / d, the culture temperature is 26 ± 1 ℃, until the growth surface produces new seed buds;

[0035] (5) When the seed buds on the growth surface are mature, they can be cut and separated from the growth surface, used as stems, and directly pl...

Embodiment 3

[0037] (1) According to the basic steps (5) described in Example 2, after the maturity of Pinellia chinensis buds on the growth surface is cut to make it break away from the growth surface, the growth surface is properly scraped (cut off the epidermis), and then pressed Step (4) described in embodiment 2, it is placed on the moisture-retaining substrate coconut shell, it is advisable to face upwards with the natural upward growth of Pinellia, cultivate under 2000lux light, the light time is 12h / d, and the cultivation temperature is 26°C. ±1°C, until the growth surface of Pinellia produces new buds;

[0038] (2) When the seed buds on the growth surface are mature, they can be cut and separated from the growth surface, and used as stems, and can be directly transplanted to the field.

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Abstract

The invention belongs to the technical field of vegetative propagation of plants, in particular to a rapid propagation method for Pinellia ternate buds. The rapid propagation method provided by the invention is characterized in that the Pinellia ternate tuber main body is subjected to wound treatment and induced to cultivate to generate a large amount of buds in a soiless substrate. The inventionprovides a rapid method of generating buds of the Pinellia ternate from tubers and seedling in the soiless substrate to grow into the tubers so as to massively produce buds for Pinellia ternate seedsor tubers for Pinellia ternate seeds in a large scale. Aiming at the characteristic of Pinellia ternate, which is in vegetative propagation manner under natural condition, the invention overcomes thedeficiencies of low propagation coefficient, long propagation period, easily degradable quality and complex process, complicated operation, strong professionalism, high investment cost in tissue culture and rapid propagation and the like, and provides a practical technology for production of buds for Pinellia ternate seeds so that the method is not only appropriate for production in a large scalefor factories, but also for self-preparation in a small scale so as to provide a novel path for supply of Pinellia ternate seed source.

Description

technical field [0001] The invention belongs to the technical field of plant asexual reproduction. In particular, it relates to a rapid propagation method of pinellia tuber, a Chinese medicinal material. Background technique [0002] Pinellia ternate. (Thunb.) Breit.) is a herbaceous plant belonging to the family Araceae. Pinellia wild resources are mainly distributed in Northeast China, North China and the Yangtze River Basin, and are mainly produced in Sichuan, Hubei, Guizhou, Henan, Anhui, Shandong, Zhejiang and other provinces. Not tolerant to drought, prefers low light and fears strong light, and loves temperature. The most suitable ground temperature is 18-20°C during the tuber bulbil expansion stage. From this point of view, the distribution area and suitable environment for growth of wild pinellia are relatively limited, the growth period is short, and the biomass is small, but the market demand is large. Pinellia is a scarce and extremely precious medicinal plant....

Claims

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Application Information

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IPC IPC(8): A01G1/00A01C1/00A01G31/00
Inventor 王沫刘良清舒少华
Owner 江西喜悦生物医药科研中心有限公司
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