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Fourth-generation HIV (Human Immunodeficiency Virus) antibody antigen test paper, preparation method and application thereof

A technology for antigen detection and antigen, which is applied in the field of fourth-generation HIV antibody antigen detection test strips and its preparation, can solve the problems of restricting the use of ordinary users, greatly affecting the detection effect of the operator's proficiency, and being inconvenient for users to use. Promotion, easy to use, simple operation effect

Active Publication Date: 2012-09-19
凡镜生物科技(山东)有限公司
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  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, some domestic manufacturers have developed the fourth-generation detection kits, mainly using chemiluminescence and enzyme-linked immunosorbent assays, but this detection method needs to be completed with the help of experimental instruments, which is inconvenient for users to use; and the detection effect It is greatly affected by the proficiency of the operator, which limits the use of ordinary users

Method used

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  • Fourth-generation HIV (Human Immunodeficiency Virus) antibody antigen test paper, preparation method and application thereof
  • Fourth-generation HIV (Human Immunodeficiency Virus) antibody antigen test paper, preparation method and application thereof
  • Fourth-generation HIV (Human Immunodeficiency Virus) antibody antigen test paper, preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Embodiment 1: the fourth generation HIV antibody antigen detection test paper (see figure 1 with figure 2 )

[0033] (1) Preparation of P24 Antigen Monoclonal Antibody

[0034] The ascites of P24 antigen monoclonal antibody was obtained by the general method of producing monoclonal antibody in animals, and then the ascites of P24 antigen monoclonal antibody was purified. After ammonium precipitation, use ultraviolet spectrometry to measure protein concentration, use SDS-PAGE to measure purity, the protein concentration of the obtained P24 antigen monoclonal antibody is higher than 2.0mg / ml, and the purity is higher than 90%.

[0035] (2) Preparation of GP36, GP41 and GP120 antigens

[0036] Precipitate the bacterial culture solution containing GP36, GP41 or GP120 antigen with caprylic acid-saturated ammonium sulfate, then purify it by ion chromatography column chromatography, and measure the protein concentration by UV spectrophotometry, and measure the purity by SD...

Embodiment 2

[0054] Embodiment 2: detection method (see image 3 , 4 、5)

[0055] Add 60 to 100 μl of the sample to be tested (whole blood, serum or plasma) directly to the sample pad 4 in the test paper obtained in Example 1 (the whole blood sample needs to add some additional diluent), and the sample will be along the reaction support 5 Each attachment moves towards the absorbent filter paper, and the experimental results are read within 20 minutes.

[0056] result:

[0057] refer to image 3 , if the sample contains the P24 antigen, it will specifically bind to the colloidal gold-labeled monoclonal antibody to the P24 antigen on the detection test paper to form a complex, and continue upward along the membrane area to form a complex with another coated nitrocellulose membrane 2 A monoclonal antibody to the P24 antigen specifically binds, and red or pink appears at the band T1; if the sample contains HIV antibodies, it is specific to the colloidal gold-labeled monoclonal antibodies t...

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Abstract

The invention provides a fourth-generation HIV (Human Immunodeficiency Virus) antibody antigen test paper, a preparation method and an application thereof. The test paper comprises a water-absorbing filter paper, a nitrocellulose membrane, a colloidal gold pad, a sample pad and a reaction supporting substance, wherein the sample pad, the colloidal gold pad, the nitrocellulose membrane and the water-absorbing filter paper are overlapped and spliced on the outer surface of the reaction supporting substance in turn. Thus, the fourth-generation HIV antibody antigen test paper provided by the invention has the advantages of convenience in use, simpleness in operation, convenience in popularization and capability of simultaneously identifying P24 antigen positive result and HIV antibody positive result on the same test paper.

Description

technical field [0001] The invention relates to the field of biological detection, in particular to a fourth-generation HIV antibody antigen detection test paper and a preparation method and application thereof. Background technique [0002] The widespread and harmfulness of HIV (Human Immunodeficiency Virus) transmission has become a hot social issue of global concern. Therefore, it is of great practical significance to improve the level of HIV diagnosis. [0003] From the advent of the first generation of HIV antibody detection reagents in 1985 to the present, HIV serological detection reagents have developed to the fourth generation. The advantage of the 4th generation ELISA reagent is that it can detect antigens and antibodies at the same time. Since the detection of antigens can shorten the window period by 7-9 days, the effect on the early diagnosis of HIV is obviously better than that of the 3rd generation. [0004] At present, some domestic manufacturers have devel...

Claims

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Application Information

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IPC IPC(8): G01N33/577G01N33/569G01N33/564G01N33/558
Inventor 顾子易江兵泽
Owner 凡镜生物科技(山东)有限公司
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